A t(14;18) kromoszóma transzlokáció kimutatása follicularis lymphomában polimeráz láncreakció segítségével.

Translated title of the contribution: Detection of t(14;18) chromosome translocation in follicular lymphoma by polymerase chain reaction

I. Takács, G. Radványi, G. Szegedi, A. Matolcsy, I. Semsei

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In most cases of centroblastic/centrocytic follicular lymphomas the bcl-2 proto-oncogene (18q21) is translocated to the immunoglobulin JH region of chromosome 14 (14q32). About three quarters of the translocations are concentrated on the 3' nontranslated, a few hundred basepare-long region of bcl-2, the so called major breakpoint region (mbr), the remaining 20-25% is located about 30 kilobases downstream of bcl-2 coding sequences in the minor cluster region (mcr). The majority of the immunoglobulin breakpoints can be found in JH6-4 genes. The polymerase chain reaction method can detect the translocation already in a very few number of cells (> 10(3)). This very sensitive technique makes it possible to detect the translocation in lymphoid/lymphoma of peripheral blood and bone marrow that are missed by other diagnostic methods. This way one can perform a quick, early diagnosis, examine the result of treatments as well as detect the remissions and the possible relapses right at the beginning. All the advantages of this method contribute to a more successful treatment of follicular lymphoma. This present work describes a polymerase chain reaction technique which is capable of a detection of the t(14;18) translocation in a patient of centroblastic/centrocytic lymphoma, moreover shows how this translocation disappears after 4 week of radiotherapy of the patient.

Original languageHungarian
Pages (from-to)1129-1132
Number of pages4
JournalOrvosi Hetilap
Volume138
Issue number18
Publication statusPublished - May 4 1997

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Chromosomes, Human, Pair 18
Chromosomes, Human, Pair 14
Follicular Lymphoma
Polymerase Chain Reaction
Immunoglobulins
Lymphoma
Proto-Oncogenes
Early Diagnosis
Radiotherapy
Cell Count
Bone Marrow
Recurrence
Therapeutics
Genes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

A t(14;18) kromoszóma transzlokáció kimutatása follicularis lymphomában polimeráz láncreakció segítségével. / Takács, I.; Radványi, G.; Szegedi, G.; Matolcsy, A.; Semsei, I.

In: Orvosi Hetilap, Vol. 138, No. 18, 04.05.1997, p. 1129-1132.

Research output: Contribution to journalArticle

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