Detection of stress and the effects of central nervous system depressants by gastrointestinal smooth muscle electromyography in wakeful rats

K. F. Szűcs, G. Grosz, M. Süle, A. Sztojkov-Ivanov, E. Ducza, A. Márki, A. Kothencz, L. Balogh, R. Gáspár

Research output: Contribution to journalArticle

Abstract

Aims: The altered gut-brain interaction can be in the background of functional gastrointestinal (GI) disorders. In the GI tract, the slow-wave myoelectric signals can be detected by electromyography (EMG). The aims of our study were to follow up the stress induced alteration in the GI tract by smooth muscle EMG in wakeful rats. Main methods: The GI tract myoelectric activity of male rats was measured by an electrode pair under the abdominal skin, the responses were detected and analyzed by a software using fast Fourier transformation. Animals were immobilized and treated with either diazepam or haloperidol. The plasma corticosterone level was determined by ELISA kit, the levels of drugs were measured by HPLC, while the direct GI effects of the compounds were tested in an organ bath. Key findings: Significant correlation (r2 = 0.52) was found between the immobilization induced increase in the EMG spectra of the GI tract segments and the increase in corticosterone plasma levels. The stress-reducing effects of diazepam and haloperidol were also detectable by smooth muscle EMG in the GI tract. No direct smooth muscle actions of the drugs were found in organ bath studies. Significance: The smooth muscle EMG instrument can measure the level of acute stress and is applicable for the investigation of central nervous system affecting drugs through the GI tract in awake rats. This is the first tool to measure the stress response via the GI tract reactions. The technique may open a new perspective in the diagnosis and therapy of psychosomatic disorders.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalLife Sciences
Volume205
DOIs
Publication statusPublished - Jul 15 2018

Fingerprint

Central Nervous System Depressants
Electromyography
Smooth Muscle
Muscle
Gastrointestinal Tract
Rats
Haloperidol
Diazepam
Corticosterone
Pharmaceutical Preparations
Baths
Plasmas
Neurology
Central Nervous System Agents
Psychophysiologic Disorders
Brain
Skin
Gastrointestinal Diseases
Animals
Immobilization

Keywords

  • Brain-gut axis
  • Corticosterone
  • Electromyography
  • Rat
  • Stress

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Detection of stress and the effects of central nervous system depressants by gastrointestinal smooth muscle electromyography in wakeful rats. / Szűcs, K. F.; Grosz, G.; Süle, M.; Sztojkov-Ivanov, A.; Ducza, E.; Márki, A.; Kothencz, A.; Balogh, L.; Gáspár, R.

In: Life Sciences, Vol. 205, 15.07.2018, p. 1-8.

Research output: Contribution to journalArticle

Szűcs, K. F. ; Grosz, G. ; Süle, M. ; Sztojkov-Ivanov, A. ; Ducza, E. ; Márki, A. ; Kothencz, A. ; Balogh, L. ; Gáspár, R. / Detection of stress and the effects of central nervous system depressants by gastrointestinal smooth muscle electromyography in wakeful rats. In: Life Sciences. 2018 ; Vol. 205. pp. 1-8.
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