Detection of siRNAs and miRNAs

Sakari Kauppinen, Zoltán Havelda

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Small RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) play crucial roles in establishing general host defense mechanisms against viral infections in plants and the development of disease symptoms. Understanding these fundamental processes requires the sensitive and specific detection of small RNA species. However, because of the small size of miRNAs and siRNAs, their detection is technically demanding. Here, we describe methods for robust and sensitive detection of small RNAs by Northern blot analysis and in situ hybridization.

Original languageEnglish
Title of host publicationPlant Virology Protocols
Subtitle of host publicationFrom Viral Sequence to Protein Function
PublisherHumana Press
Pages217-227
Number of pages11
ISBN (Print)9781588298270
DOIs
Publication statusPublished - Jan 1 2008

Publication series

NameMethods in Molecular Biology
Volume451
ISSN (Print)1064-3745

Keywords

  • Detection
  • In situ hybridization
  • LNA
  • MiRNA
  • Northern blot
  • SiRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Kauppinen, S., & Havelda, Z. (2008). Detection of siRNAs and miRNAs. In Plant Virology Protocols: From Viral Sequence to Protein Function (pp. 217-227). (Methods in Molecular Biology; Vol. 451). Humana Press. https://doi.org/10.1007/978-1-59745-102-4_15