Detection of self-complementary inverted repeats by single forward primer driven PCR

Tünde Kupi, Tamás Deák, György D. Bisztray, E. Szegedi

Research output: Contribution to journalArticle

Abstract

Inverted repeat gene structures designed for silencing functional genes have been widely used both in academic and applied research. The correct orientations of such structures are usually validated with restriction analysis and/or sequencing. We speculated that the inverted repeat nature of such constructs can be shown by a simple PCR reaction with a single forward primer. To test this hypothesis five different constructs were established from grapevine sequences in a hairpin-intron style silencing system. We were able to amplify the appropriate products in each case. Thus a forward-primed PCR alone may be sufficient to prove the inverted repeat nature of the desired constructs.

Original languageEnglish
Pages (from-to)65-68
Number of pages4
JournalActa Biologica Szegediensis
Volume58
Issue number1
Publication statusPublished - 2014

Fingerprint

Genes
Polymerase Chain Reaction
Gene Silencing
Introns
introns
genes
Research
testing

Keywords

  • Agrobacterium tumefaciens
  • Crown gall
  • Phire taq polymerase
  • Plant diseases
  • RNA interference

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Detection of self-complementary inverted repeats by single forward primer driven PCR. / Kupi, Tünde; Deák, Tamás; Bisztray, György D.; Szegedi, E.

In: Acta Biologica Szegediensis, Vol. 58, No. 1, 2014, p. 65-68.

Research output: Contribution to journalArticle

Kupi, Tünde ; Deák, Tamás ; Bisztray, György D. ; Szegedi, E. / Detection of self-complementary inverted repeats by single forward primer driven PCR. In: Acta Biologica Szegediensis. 2014 ; Vol. 58, No. 1. pp. 65-68.
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