Detection of previously unknown fumonisin P analogue mycotoxins in a fusarium verticillioides culture by high-performance liquid chromatography- electrospray ionization time-of-flight and ion trap mass spectrometry

Tibor Bartók, László Tölgyesi, Árpád Szécsi, Ákos Mesterházy, Mihály Bartók, Erno Gyimes, Antal Véha

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Five previously unknown fumonisin mycotoxins (iso-FP1, iso-FP2,3a, iso-FP2,3b, FP4 and iso-FP 4) and three previously described FP analogues (FP1-3) were detected in a solid rice culture inoculated with Fusarium verticillioides. The fumonisins were characterized by high-performance liquid chromatography-electrospray ionization time-of-flight mass spectrometry (HPLC-ESI-TOFMS) and ion trap mass spectrometry (ITMS). The FP isomers were separated by using a flat gradient on a special, high-coverage C18, narrow-bore HPLC column (YMC-Pack J'sphere ODS H80), which was suggested for the separation of structural isomers. The verified structures of the FP1-3 mycotoxins, the relative retention times (by HPLC-ESI-TOFMS and ITMS), the exact masses of the molecular ions (by TOFMS) and the masses of the product ions, including the hydrocarbon backbones (by ITMS) of the new compounds, strongly suggested their structures.

Original languageEnglish
Pages (from-to)508-513
Number of pages6
JournalJournal of chromatographic science
Volume52
Issue number6
DOIs
Publication statusPublished - Jul 2014

ASJC Scopus subject areas

  • Analytical Chemistry

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