Detection of osteoprotegerin and TNF-alpha mRNA in ankylotic stapes footplates in connection with measles virus positivity

T. Karosi, István Jókay, József Kónya, László Z. Szabó, József Pytel, J. Jóri, Anita Szalmás, I. Sziklai

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

HYPOTHESIS: Otosclerosis is a bone remodeling disorder of the otic capsule causing conductive and sensorineural hearing loss. Persistent measles virus infection of the temporal bone with increased tumor necrosis factor (TNF)-alpha and decreased osteoprotegerin mRNA expression is supposed to be the main etiologic factor in otosclerosis. BACKGROUND: Determinants of measles virus infection and reactive inflammation were studied in otosclerosis. The presence of measles virus was shown in otosclerotic patients by reverse transcriptase-polymerase chain reaction (RT-PCR) amplification of the viral RNA. No report is available, however, about the role and interactions of bone-specific cytokines in otosclerosis. METHODS: Nucleic acid was extracted from stapes footplates of clinically otosclerotic patients. Measles virus nucleoprotein RNA was amplified by seminested RT-PCR. TNF-alpha and osteoprotegerin mRNA coexpression was detected by RT-PCR in otosclerotic bone and was correlated to measles virus positivity. RESULTS: Among 154 clinically stapes fixation otosclerotic patients, 99 stapedes contained measles virus RNA. TNF-alpha mRNA was detectable in 88 virus-positive and in 6 virus-negative stapes footplates. Osteoprotegerin mRNA expression was significantly lower in the TNF-alpha-positive specimens (P <.0001) that was independent from virus positivity. CONCLUSION: Detection of TNF-alpha mRNA demonstrates activated osteoclast functions and inflammatory pathways in otosclerotic stapes footplates associated with measles virus presence. Increased expression of TNF-alpha and its action on RANK production inhibits the protective functions of osteoprotegerin on normal bone turnover in the otic capsule.

Original languageEnglish
Pages (from-to)1427-1433
Number of pages7
JournalLaryngoscope
Volume116
Issue number8
DOIs
Publication statusPublished - Aug 2006

Fingerprint

Stapes
Osteoprotegerin
Measles virus
Otosclerosis
Tumor Necrosis Factor-alpha
Messenger RNA
Reverse Transcriptase Polymerase Chain Reaction
Bone Remodeling
Virus Diseases
Viruses
Capsules
Ear
RNA
Conductive Hearing Loss
Bone and Bones
Sensorineural Hearing Loss
Temporal Bone
Viral RNA
Osteoclasts
Nucleic Acids

Keywords

  • Measles virus
  • Osteoprotegerin
  • Otosclerosis
  • RT-PCR
  • Stapes fixation
  • TNF-alpha

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Detection of osteoprotegerin and TNF-alpha mRNA in ankylotic stapes footplates in connection with measles virus positivity. / Karosi, T.; Jókay, István; Kónya, József; Szabó, László Z.; Pytel, József; Jóri, J.; Szalmás, Anita; Sziklai, I.

In: Laryngoscope, Vol. 116, No. 8, 08.2006, p. 1427-1433.

Research output: Contribution to journalArticle

Karosi, T. ; Jókay, István ; Kónya, József ; Szabó, László Z. ; Pytel, József ; Jóri, J. ; Szalmás, Anita ; Sziklai, I. / Detection of osteoprotegerin and TNF-alpha mRNA in ankylotic stapes footplates in connection with measles virus positivity. In: Laryngoscope. 2006 ; Vol. 116, No. 8. pp. 1427-1433.
@article{242aaacaf31947df9b298e2a1a4cae5e,
title = "Detection of osteoprotegerin and TNF-alpha mRNA in ankylotic stapes footplates in connection with measles virus positivity",
abstract = "HYPOTHESIS: Otosclerosis is a bone remodeling disorder of the otic capsule causing conductive and sensorineural hearing loss. Persistent measles virus infection of the temporal bone with increased tumor necrosis factor (TNF)-alpha and decreased osteoprotegerin mRNA expression is supposed to be the main etiologic factor in otosclerosis. BACKGROUND: Determinants of measles virus infection and reactive inflammation were studied in otosclerosis. The presence of measles virus was shown in otosclerotic patients by reverse transcriptase-polymerase chain reaction (RT-PCR) amplification of the viral RNA. No report is available, however, about the role and interactions of bone-specific cytokines in otosclerosis. METHODS: Nucleic acid was extracted from stapes footplates of clinically otosclerotic patients. Measles virus nucleoprotein RNA was amplified by seminested RT-PCR. TNF-alpha and osteoprotegerin mRNA coexpression was detected by RT-PCR in otosclerotic bone and was correlated to measles virus positivity. RESULTS: Among 154 clinically stapes fixation otosclerotic patients, 99 stapedes contained measles virus RNA. TNF-alpha mRNA was detectable in 88 virus-positive and in 6 virus-negative stapes footplates. Osteoprotegerin mRNA expression was significantly lower in the TNF-alpha-positive specimens (P <.0001) that was independent from virus positivity. CONCLUSION: Detection of TNF-alpha mRNA demonstrates activated osteoclast functions and inflammatory pathways in otosclerotic stapes footplates associated with measles virus presence. Increased expression of TNF-alpha and its action on RANK production inhibits the protective functions of osteoprotegerin on normal bone turnover in the otic capsule.",
keywords = "Measles virus, Osteoprotegerin, Otosclerosis, RT-PCR, Stapes fixation, TNF-alpha",
author = "T. Karosi and Istv{\'a}n J{\'o}kay and J{\'o}zsef K{\'o}nya and Szab{\'o}, {L{\'a}szl{\'o} Z.} and J{\'o}zsef Pytel and J. J{\'o}ri and Anita Szalm{\'a}s and I. Sziklai",
year = "2006",
month = "8",
doi = "10.1097/01.mlg.0000225928.35838.e5",
language = "English",
volume = "116",
pages = "1427--1433",
journal = "Laryngoscope",
issn = "0023-852X",
publisher = "John Wiley and Sons Inc.",
number = "8",

}

TY - JOUR

T1 - Detection of osteoprotegerin and TNF-alpha mRNA in ankylotic stapes footplates in connection with measles virus positivity

AU - Karosi, T.

AU - Jókay, István

AU - Kónya, József

AU - Szabó, László Z.

AU - Pytel, József

AU - Jóri, J.

AU - Szalmás, Anita

AU - Sziklai, I.

PY - 2006/8

Y1 - 2006/8

N2 - HYPOTHESIS: Otosclerosis is a bone remodeling disorder of the otic capsule causing conductive and sensorineural hearing loss. Persistent measles virus infection of the temporal bone with increased tumor necrosis factor (TNF)-alpha and decreased osteoprotegerin mRNA expression is supposed to be the main etiologic factor in otosclerosis. BACKGROUND: Determinants of measles virus infection and reactive inflammation were studied in otosclerosis. The presence of measles virus was shown in otosclerotic patients by reverse transcriptase-polymerase chain reaction (RT-PCR) amplification of the viral RNA. No report is available, however, about the role and interactions of bone-specific cytokines in otosclerosis. METHODS: Nucleic acid was extracted from stapes footplates of clinically otosclerotic patients. Measles virus nucleoprotein RNA was amplified by seminested RT-PCR. TNF-alpha and osteoprotegerin mRNA coexpression was detected by RT-PCR in otosclerotic bone and was correlated to measles virus positivity. RESULTS: Among 154 clinically stapes fixation otosclerotic patients, 99 stapedes contained measles virus RNA. TNF-alpha mRNA was detectable in 88 virus-positive and in 6 virus-negative stapes footplates. Osteoprotegerin mRNA expression was significantly lower in the TNF-alpha-positive specimens (P <.0001) that was independent from virus positivity. CONCLUSION: Detection of TNF-alpha mRNA demonstrates activated osteoclast functions and inflammatory pathways in otosclerotic stapes footplates associated with measles virus presence. Increased expression of TNF-alpha and its action on RANK production inhibits the protective functions of osteoprotegerin on normal bone turnover in the otic capsule.

AB - HYPOTHESIS: Otosclerosis is a bone remodeling disorder of the otic capsule causing conductive and sensorineural hearing loss. Persistent measles virus infection of the temporal bone with increased tumor necrosis factor (TNF)-alpha and decreased osteoprotegerin mRNA expression is supposed to be the main etiologic factor in otosclerosis. BACKGROUND: Determinants of measles virus infection and reactive inflammation were studied in otosclerosis. The presence of measles virus was shown in otosclerotic patients by reverse transcriptase-polymerase chain reaction (RT-PCR) amplification of the viral RNA. No report is available, however, about the role and interactions of bone-specific cytokines in otosclerosis. METHODS: Nucleic acid was extracted from stapes footplates of clinically otosclerotic patients. Measles virus nucleoprotein RNA was amplified by seminested RT-PCR. TNF-alpha and osteoprotegerin mRNA coexpression was detected by RT-PCR in otosclerotic bone and was correlated to measles virus positivity. RESULTS: Among 154 clinically stapes fixation otosclerotic patients, 99 stapedes contained measles virus RNA. TNF-alpha mRNA was detectable in 88 virus-positive and in 6 virus-negative stapes footplates. Osteoprotegerin mRNA expression was significantly lower in the TNF-alpha-positive specimens (P <.0001) that was independent from virus positivity. CONCLUSION: Detection of TNF-alpha mRNA demonstrates activated osteoclast functions and inflammatory pathways in otosclerotic stapes footplates associated with measles virus presence. Increased expression of TNF-alpha and its action on RANK production inhibits the protective functions of osteoprotegerin on normal bone turnover in the otic capsule.

KW - Measles virus

KW - Osteoprotegerin

KW - Otosclerosis

KW - RT-PCR

KW - Stapes fixation

KW - TNF-alpha

UR - http://www.scopus.com/inward/record.url?scp=33746850628&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746850628&partnerID=8YFLogxK

U2 - 10.1097/01.mlg.0000225928.35838.e5

DO - 10.1097/01.mlg.0000225928.35838.e5

M3 - Article

VL - 116

SP - 1427

EP - 1433

JO - Laryngoscope

JF - Laryngoscope

SN - 0023-852X

IS - 8

ER -