Detection of ochratoxin A in aptamer assay using total internal reflection ellipsometry

Ali Al Rubaye, Alexei Nabok, Gaelle Catanante, Jean Louis Marty, Ezster Takacs, A. Székács

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The current work is a continuation of our research targeted the development of novel optical sensing technologies for detection of mycotoxins. The method of (TIRE) was developed in the last decade as a combination of spectroscopic ellisometry and SPR and was proved to be a highly sensitive analytical tool in bio-sensing particularly attractive for detection of low molecular weight analytes, such as mycotoxins. The use of aptamers as highly specific artificial molecular receptors to ochratoxin A (OTA) in conjunction with the method Total Internal Reflection Ellipsometry (TIRE) is reported here for the first time. Our results showed a possibility of label-free optical detection of OTA down to 0.01 ppb in concentration in direct assay with specific aptamer. The kinetics of aptamer/OTA binding was studied with dynamic TIRE spectral measurements and allowed evaluating the affinity constant KD = 1.8 10−8 Mol which is characteristic for highly specific aptamer/OTA binding.

Original languageEnglish
Pages (from-to)248-251
Number of pages4
JournalSensors and Actuators, B: Chemical
Volume263
DOIs
Publication statusPublished - Jun 15 2018

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Keywords

  • Aptamer
  • Binding kinetics
  • Label-free optical biosensor
  • Ochratoxin A
  • Total internal reflection ellipsometry

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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