Detection of non-polio enteroviruses in Hungary 2000-2008 and molecular epidemiology of enterovirus 71, coxsackievirus A16, and echovirus 30

Beatrix Kapusinszky, Katalin N. Szomor, Ágnes Farkas, Mária Takács, György Berencsi

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Human enteroviruses are associated with various clinical syndromes from minor febrile illness to severe, potentially fatal conditions like aseptic meningitis, paralysis, myocarditis, and neonatal enteroviral sepsis. Between June 2000 and August 2008 echovirus (E) type 2, 4, 6, 7, 9, 11, 13, 25, 30, coxsackievirus (CV) -A16, -A19, -B5, and enterovirus 71 (EV71) were reported in Hungary. In this study, 29 previously enterovirus positive samples from 28 patients diagnosed with hand, foot and mouth disease, meningitis and encephalitis, were molecularly typed. The genetic relationships of identified serotypes CV-A16, EV71, and E30 were assessed by direct sequencing of genomic region encoding the capsid protein VP1. The sequences were compared to each other and sequences from other geographical regions possessed in Genbank. The phylogenetic analysis of CV-A16 revealed that the viruses were mostly of Far-Eastern or Asia-Pacific origin. Typing of EV71 showed that one virus from 2000 belonged to genotype C1 and five viruses observed in 2004 and 2005 were identified as genotype C4. The 11 echovirus 30 strains showed homology with those of neighbor European countries. The molecular examination of E30 revealed that three separate lineages circulated in 2000, 2001, and 2004- 2006 in Hungary.

Original languageEnglish
Pages (from-to)163-173
Number of pages11
JournalVirus Genes
Issue number2
Publication statusPublished - Apr 1 2010



  • Echovirus 30 and enterovirus 71
  • Foot and mouth disease
  • Genetic clusters of coxsackievirus A16
  • Hand
  • Meningitis and encephalitis
  • Non-polio enteroviruses

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Virology

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