The authors have tested 3 primer pairs specific for M. bovis genome by using PCR technique. They have determined the optimal reaction conditions for the three PCR systems. They have tested the sensitivity and efficacy by using clinical samples originating from naturally infected cows and calves, artificially challenged calves and broth cultures. DNA was prepared from clinical samples cultured in broth medium for 24-48 hours. Before the challenge, 1-3% of the animals were positive for M. bovis, however, only the M. bovis negative animals were challenged. Three weeks after the intranasal challenge M. bovis was isolated in about 60% of samples, taken from nasal swabs, lung-lavage, lung tissues, peribronchial lymph nodes and inner organs. M. bovis was detected more frequently by PCR than by culture. Using all three-primer pairs, they have compared one Hungarian M. bovis strain and 4 M. bovis strains of international origin, showing different virulence in challenge experiments. All three PCR primer pairs reacted with all of the strains, producing amplicons characteristics of each primer pairs, however, the use of uvrC primer provided the most sensitive system.
|Translated title of the contribution||Detection of Mycoplasma bovis by different PCR systems|
|Number of pages||5|
|Journal||Magyar Allatorvosok Lapja|
|Publication status||Published - Oct 1 2004|
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