Detection of microRNAs in plants by in situ hybridisation.

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are short, about 21 nucleotides in length, non-coding, regulatory RNA molecules representing a new layer in post-transcriptional gene expression regulation. Spatial and temporal analysis of miRNA accumulation by in situ analyses is the prerequisite of understanding the precise biological functions of miRNAs. Since miRNAs are very short molecules, their in situ analysis is technically demanding. Our method is based on the usage of highly sensitive LNA-modified oligonucleotide probes. LNA modification significantly enhances the sensitivity and specificity of miRNA detecting probes and provides relatively easy in situ miRNA detection. Here, we describe a protocol for this challenging technique step by step, in order to help every user to achieve success.

Original languageEnglish
Pages (from-to)9-23
Number of pages15
JournalMethods in molecular biology (Clifton, N.J.)
Volume732
Publication statusPublished - 2011

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MicroRNAs
In Situ Hybridization
Spatio-Temporal Analysis
Untranslated RNA
Oligonucleotide Probes
Gene Expression Regulation
Nucleotides
Sensitivity and Specificity

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Detection of microRNAs in plants by in situ hybridisation. / Várallyay, E.; Havelda, Z.

In: Methods in molecular biology (Clifton, N.J.), Vol. 732, 2011, p. 9-23.

Research output: Contribution to journalArticle

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