The frequency of methylenetetrahydrofolate reductase C677T polymorphism has not been studied in patients with HELLP syndrome. We decided to determine it in Hungarian severe preeclamptic and HELLP syndrome patients. We determined the distribution of the MTHFR C677T mutation in DNA samples of 73 healthy pregnant, 101 severe preeclamptic and 63 HELLP syndrome effected women using the quantitative real-time PCR method. We found a significantly higher number of TT genotype (25.4%) in the HELLP syndrome group as compared to the healthy (8.2%) and also in the severe preeclamptics (8.9%)(p=0.03). However, we found almost the same genotype distribution in the healthy pregnant and preeclamptic groups. The distribution of the C and T alleles showed the same phenomenon. The frequency of the mutant T allele was 45.2% in HELLP syndrome, while it was 32.2% in the healthy pregnant (p=0.03), and 30.2% (p=0.008) in the severe preeclamptic patients. We detected a higher frequency of MTHFR C677T mutation in HELLP syndrome patients, using the quantitative real-time PCR method. It can explain the premature vascular disease and venous thrombosis observed in these patients. Determination of MTHFR genotype and the measurement of homocystein levels could be a helpful information in the management of patients having preeclampsia and HELLP syndrome.
|Translated title of the contribution||Detection of methylenetetrahydrofolate reductase (MTHFR) C677T mutation by quantitative real-time PCR method in patients with HELLP syndrome|
|Number of pages||5|
|Journal||Magyar Noorvosok Lapja|
|Publication status||Published - Jun 14 2007|
ASJC Scopus subject areas
- Reproductive Medicine
- Obstetrics and Gynaecology