Detection of maternal deoxyribonucleic acid in peripheral blood of premature and mature newborn infants

Levente Lázár, Ágnes Harmath, Zoltán Bán, Bálint Nagy, Csaba Papp, János Rigó, Zoltán Papp

Research output: Contribution to journalArticle

2 Citations (Scopus)


Background: Over the past decade, a lot of attention has been directed towards the fetomaternal and maternofetal transfer of nucleated cells and plasma DNA. In some autoimmune diseases, the fetal DNA is suspected to play an important role in the etiology of the disease. In the same way, the presence of maternal cells and free plasma DNA in fetal/newborn circulation gives rise to interesting questions. The aim of our study was to detect maternal deoxyribonucleic acid in the peripheral blood of premature and mature newborn infants. Methods: In the case of eight RhD-positive mothers-RhD-negative newborn pairs, peripheral blood samples were collected from the newborn infants within 35-120 min after birth. The maternal origin DNA was determined by real-time PCR amplification of the exon 7 of the RhD-positive allele. Result: In all eight cases, the RhD exon 7 was amplified during the PCR reaction. Conclusion: The result of our study demonstrates that maternal DNA is present in the fetal peripheral circulation. The presence of maternally derived cells/DNA in the blood of newborn infants might have a role in the immunization of the newborn infants and also could be a possible explanation for 'grandmother effect' in the case of Rh-negative nulligravida patients.

Original languageEnglish
Pages (from-to)168-170
Number of pages3
JournalPrenatal Diagnosis
Issue number2
Publication statusPublished - Feb 1 2006


  • Maternal DNA
  • Peripheral blood
  • Real-time PCR
  • RhD status

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Genetics(clinical)

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