Detection of Lawsonia intracellularis in Hungarian swine herds by polymerase chain reaction

I. Biksi, I. Kacskovics, Mira Mákdoki, Judit Iván, I. Horváth-Papp, G. Makay, F. Vetési

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8 Citations (Scopus)

Abstract

Pooled faecal samples and/or intestinal contents from pig carcasses were collected from 11 Hungarian swine farms and subjected to DNA extraction/ purification and subsequent polymerase chain reaction (PCR) in order to detect Lawsonia intracellularis, the aetiological agent of the porcine proliferative enteropathy complex. Specific PCR positivity was detected in 6 individual and 22 pooled samples out of 46, originating from eight herds. The PCR products of collected faecal samples hybridised in Southern blot hybridisation with the DNA of the type strain L. intracellularis NCTC 12657. This is the first confirmed detection of L. intracellularis by PCR in Hungary.

Original languageEnglish
Pages (from-to)415-420
Number of pages6
JournalActa veterinaria Hungarica
Volume46
Issue number4
Publication statusPublished - Dec 1 1998

Keywords

  • Lawsonia intracellularis
  • PCR
  • Porcine proliferative enteropathy complex
  • Southern blot hybridisation

ASJC Scopus subject areas

  • veterinary(all)

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    Biksi, I., Kacskovics, I., Mákdoki, M., Iván, J., Horváth-Papp, I., Makay, G., & Vetési, F. (1998). Detection of Lawsonia intracellularis in Hungarian swine herds by polymerase chain reaction. Acta veterinaria Hungarica, 46(4), 415-420.