A fertozo bronchitis vírus, a csirke nephritis vírus és a fertozo bursitis vírus kimutatása multiplex RT-PCR alapú diagnosztikai eljárással

Translated title of the contribution: Detection of infectious bronchitis virus, avian nephritis virus and infectious bursal disease virus by multiplex RT-PCR based diagnostic method

Palade Elena Alina, Demeter Zoltán, M. Dobos-Kovács, M. Rusvai, Mándoki Míra

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Nephroso-nephritis and uricosis are known to cause massive production loss in the chicken industry. Beside the various causes of non infective nature, leading to regressive renal changes, there are viral infections frequently related with regressive and inflammatory kidney lesions in birds. The best known causative agents are infectious bronchitis virus (IBV) and avian nephritis virus (ANV), but renal changes were also reported in case of infectious bursal disease virus (IBDV) infections. The authors present a diagnostic method based on multiplex polymerase chain reaction (mPCR) following reverse transcription, to identify and hence discriminate the infections caused by the three pathogens in one reaction, and so obtaining a faster, cheaper, but still reliable result. The used primer pairs amplify gel separable products from highly conserved region of the nucleocapsid (N) protein for IBV, viral protein 2 (VP2) for IBDV and glycoprotein 1 (GP1) protein for ANV, respectively. The mPCR products were visualized by gel electrophoresis and were represented by fragments of 743 base pairs (bp) for IBVD, 608 bp for ANV and 402 bp for IBV. When testing the sensitivity of the newly developed diagnostic test by using a serially diluted positive control with known concentration of genetic material, the multiplex PCR could detect 8.45 × 10-2 μg for IBDV, 8.87 × 10 -3 μg for IBV, and 8.92 × 10-3 μg for ANV, respectively, indicating that it is a rapid and sensitive diagnostic method, saving time and effort without compromising the diagnostic purpose.

Original languageHungarian
Pages (from-to)559-564
Number of pages6
JournalMagyar Allatorvosok Lapja
Volume130
Issue number9
Publication statusPublished - 2008

Fingerprint

Avastrovirus
Avian nephritis virus
Infectious bursal disease virus
Infectious bronchitis virus
Multiplex Polymerase Chain Reaction
diagnostic techniques
reverse transcriptase polymerase chain reaction
Base Pairing
kidneys
Virus Diseases
Kidney
Gels
polymerase chain reaction
infection
Nucleocapsid Proteins
nucleocapsid
nephritis
reverse transcription
Nephritis
viral proteins

ASJC Scopus subject areas

  • veterinary(all)

Cite this

A fertozo bronchitis vírus, a csirke nephritis vírus és a fertozo bursitis vírus kimutatása multiplex RT-PCR alapú diagnosztikai eljárással. / Alina, Palade Elena; Zoltán, Demeter; Dobos-Kovács, M.; Rusvai, M.; Míra, Mándoki.

In: Magyar Allatorvosok Lapja, Vol. 130, No. 9, 2008, p. 559-564.

Research output: Contribution to journalArticle

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abstract = "Nephroso-nephritis and uricosis are known to cause massive production loss in the chicken industry. Beside the various causes of non infective nature, leading to regressive renal changes, there are viral infections frequently related with regressive and inflammatory kidney lesions in birds. The best known causative agents are infectious bronchitis virus (IBV) and avian nephritis virus (ANV), but renal changes were also reported in case of infectious bursal disease virus (IBDV) infections. The authors present a diagnostic method based on multiplex polymerase chain reaction (mPCR) following reverse transcription, to identify and hence discriminate the infections caused by the three pathogens in one reaction, and so obtaining a faster, cheaper, but still reliable result. The used primer pairs amplify gel separable products from highly conserved region of the nucleocapsid (N) protein for IBV, viral protein 2 (VP2) for IBDV and glycoprotein 1 (GP1) protein for ANV, respectively. The mPCR products were visualized by gel electrophoresis and were represented by fragments of 743 base pairs (bp) for IBVD, 608 bp for ANV and 402 bp for IBV. When testing the sensitivity of the newly developed diagnostic test by using a serially diluted positive control with known concentration of genetic material, the multiplex PCR could detect 8.45 × 10-2 μg for IBDV, 8.87 × 10 -3 μg for IBV, and 8.92 × 10-3 μg for ANV, respectively, indicating that it is a rapid and sensitive diagnostic method, saving time and effort without compromising the diagnostic purpose.",
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