Detection of immune complexes by matrix-assisted laser desorption/ionization mass spectrometry

G. Schlosser, Gabriella Pocsfalvi, Antonio Malorni, Angel Puerta, Mercedes De Frutos, K. Vékey

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was used to detect an immune complex formed between β-lactoglobulin and polyclonal anti-β-lactoglobulin antibody in the gas phase. The most important experimental parameters to detect such a specific antibody-antigen complex by MALDI were the use of solutions at near-neutral pH and of sinapinic acid matrix prepared by the dried-droplet method. Under such conditions, predominantly one but also two molecules of antigen protein were complexed by the antibody. Specific formation of the antibody-antigen complex was confirmed by performing competitive reactions. Addition of antibody to a 1:1 mixture of β-lactoglobulin and one control protein resulted not only in the appearance of the expected antibody-antigen complex, but also in a strong decrease in the free β-lactoglobulin signal, while the abundance of the control protein was not influenced.

Original languageEnglish
Pages (from-to)2741-2747
Number of pages7
JournalRapid Communications in Mass Spectrometry
Volume17
Issue number24
Publication statusPublished - 2003

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Lactoglobulins
Antigen-Antibody Complex
Ionization
Mass spectrometry
Desorption
Lasers
Antibodies
Proteins
Gases
Antigens
Molecules

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

Detection of immune complexes by matrix-assisted laser desorption/ionization mass spectrometry. / Schlosser, G.; Pocsfalvi, Gabriella; Malorni, Antonio; Puerta, Angel; De Frutos, Mercedes; Vékey, K.

In: Rapid Communications in Mass Spectrometry, Vol. 17, No. 24, 2003, p. 2741-2747.

Research output: Contribution to journalArticle

Schlosser, G. ; Pocsfalvi, Gabriella ; Malorni, Antonio ; Puerta, Angel ; De Frutos, Mercedes ; Vékey, K. / Detection of immune complexes by matrix-assisted laser desorption/ionization mass spectrometry. In: Rapid Communications in Mass Spectrometry. 2003 ; Vol. 17, No. 24. pp. 2741-2747.
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