Detection of immune complexes by matrix-assisted laser desorption/ionization mass spectrometry

Gitta Schlosser, Gabriella Pocsfalvi, Antonio Malorni, Angel Puerta, Mercedes De Frutos, Károly Vékey

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was used to detect an immune complex formed between β-lactoglobulin and polyclonal anti-β-lactoglobulin antibody in the gas phase. The most important experimental parameters to detect such a specific antibody-antigen complex by MALDI were the use of solutions at near-neutral pH and of sinapinic acid matrix prepared by the dried-droplet method. Under such conditions, predominantly one but also two molecules of antigen protein were complexed by the antibody. Specific formation of the antibody-antigen complex was confirmed by performing competitive reactions. Addition of antibody to a 1:1 mixture of β-lactoglobulin and one control protein resulted not only in the appearance of the expected antibody-antigen complex, but also in a strong decrease in the free β-lactoglobulin signal, while the abundance of the control protein was not influenced.

Original languageEnglish
Pages (from-to)2741-2747
Number of pages7
JournalRapid Communications in Mass Spectrometry
Volume17
Issue number24
DOIs
Publication statusPublished - Jan 1 2003

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Detection of immune complexes by matrix-assisted laser desorption/ionization mass spectrometry'. Together they form a unique fingerprint.

  • Cite this