Detection of hydrogen peroxide by lactoperoxidase-mediated dityrosine formation

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Abstract

The aim of this work was to study the dityrosine-forming activity of lactoperoxidase (LPO) and its potential application for measuring hydrogen peroxide (H2O2). It was observed that LPO was able to form dityrosine at low H2O2 concentrations. Since dityrosine concentration could be measured in a simple fluorimetric reaction, this activity of the enzyme was utilized for the measurement of H2O2 production in different systems. These experiments successfully measured the activity of NADPH oxidase 4 (Nox4) by this method. It was concluded that LPO-mediated dityrosine formation offers a simple way for H2 O2 measurement.

Original languageEnglish
Pages (from-to)440-445
Number of pages6
JournalFree Radical Research
Volume43
Issue number5
DOIs
Publication statusPublished - Jul 20 2009

Keywords

  • Dityrosine
  • Hydrogen peroxide
  • Lactoperoxidase
  • NADPH oxidase 4
  • Reactive oxygen species

ASJC Scopus subject areas

  • Biochemistry

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