Humán papillomavírus génszakaszok kimutatása laryngealis daganatokban és praemalignus elváltozásokban polimeráz láncreakcióval.

Translated title of the contribution: Detection of human papillomavirus gene sequences in laryngeal tumors and premalignant changes by polymerase chain reaction

J. Czeglédy, T. Major, A. Juhász, G. Répássy, L. Gergely

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Human papillomavirus gene sequences have been detected in a number of malignant and benign tumours. Non-oncogenic types 6 and 11 are etiological factors of benign mucosal tumours. Types 16 and 18 can be detected in malignancies most often but their role in the etiopathogenesis of cancers is still unclear. In our study we examined formalin-fixed and paraffin-embedded archive laryngeal tissues containing squamous cell carcinoma, papilloma and precancerous lesions for the presence of human papillomavirus genes. As a control we also examined tissues harbouring laryngeal nodules which represented the normal larynx in our study. After DNA preparation from the paraffin blocks we performed polymerase chain reaction to detect the DNA of human papillomavirus types 6, 11, 16 and 18. In the squamous cell carcinomas, papillomas and precancerous lesions the presence of human papillomavirus gene sequences was significantly higher than in the control group. To verify the integrity of DNA we also amplified a sequence deriving from the cellular beta-globin gene. Based on the 100% positivity for this gene we declare that the combination of our DNA preparation and polymerase chain reaction is a reliable method for detecting DNA sequences from formalin-fixed and paraffin-embedded tissues.

Original languageHungarian
Pages (from-to)1891-1895
Number of pages5
JournalOrvosi Hetilap
Volume138
Issue number30
Publication statusPublished - Jul 27 1997

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Polymerase Chain Reaction
Paraffin
Genes
Papilloma
Neoplasms
Formaldehyde
Squamous Cell Carcinoma
DNA
Human papillomavirus 11
Human papillomavirus 6
Human papillomavirus 18
beta-Globins
DNA-Directed DNA Polymerase
Larynx
Control Groups

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Humán papillomavírus génszakaszok kimutatása laryngealis daganatokban és praemalignus elváltozásokban polimeráz láncreakcióval. / Czeglédy, J.; Major, T.; Juhász, A.; Répássy, G.; Gergely, L.

In: Orvosi Hetilap, Vol. 138, No. 30, 27.07.1997, p. 1891-1895.

Research output: Contribution to journalArticle

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