Detection of human papillomavirus deoxyribonucleic acid in the female genital tract

J. Czeglédy, L. Gergely, Z. Hernádi, R. Póka

Research output: Contribution to journalArticle

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Abstract

A total of 336 biopsies, scrapes and exfoliated cells from the cervix and from the lower genital tract were screened for human papilloma (HP) viral sequences of types 6, 11, 16 and 18 by Southern blot, dot blot and filter in situ (FISH) hybridizations with cloned 32P-radiolabeled HPV DNA probes. The specimens included cervical intraepithelial neoplasias (CIN I-III), carcinoma in situ and invasive carcinoma of the cervix and vagina, adenocarcinomas, vulvar and vaginal condylomata acuminata and healthy epithelial samples. The oncogenic HPV 16 was found in 46% of the cervical carcinomas. Most of the type 16 occurences (75%) represented the third stage of inooperable cases. Similarly, HPV 18 was also most frequently present in this stage as well as in carcinoma in situ and in CIN III (25%, 18%). At the same time, in condylomata acuminata, types 6 and 11 were detectable in 88.7% of cares. In all, 13.5% of the normal samples harboured HPV DNA.

Original languageEnglish
Pages (from-to)309-314
Number of pages6
JournalMedical Microbiology and Immunology
Volume178
Issue number6
DOIs
Publication statusPublished - Nov 1989

Fingerprint

Condylomata Acuminata
Carcinoma in Situ
HPV DNA Probes
Cervix Uteri
Carcinoma
Human papillomavirus 18
Cervical Intraepithelial Neoplasia
Human papillomavirus 16
DNA
Papilloma
Vagina
Southern Blotting
In Situ Hybridization
Adenocarcinoma
Biopsy

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology and Allergy
  • Immunology

Cite this

Detection of human papillomavirus deoxyribonucleic acid in the female genital tract. / Czeglédy, J.; Gergely, L.; Hernádi, Z.; Póka, R.

In: Medical Microbiology and Immunology, Vol. 178, No. 6, 11.1989, p. 309-314.

Research output: Contribution to journalArticle

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