Detection of human cytomegalovirus-induced DNA-binding proteins on Western blots

Lajos Gergely, Judit Czegledy, Lajos Váczi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

DNA-binding proteins have been isolated from human embryonic fibroblast cells infected with human cytomegalovirus strain AD169 by native and denatured DNA-cellulose affinity chromatography and Polymin P precipitation. The DNA-binding proteins separated by polyacrylamide gel electrophoresis and electrophoretically transferred to a nitrocellulose sheet were identified by a modified ELISA reaction. Six DNA-binding proteins were found in infected cells, their molecular weights range from 52K to 18K.

Original languageEnglish
Pages (from-to)263-270
Number of pages8
JournalJournal of Virological Methods
Volume12
Issue number3-4
DOIs
Publication statusPublished - Dec 1985

Keywords

  • DNA-binding proteins
  • Western blotting
  • cytomegalovirus

ASJC Scopus subject areas

  • Virology

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