Detection of homologous DNA sequences in animal adenoviruses by polymerase chain reaction

I. Kiss, Katalin Matiz, Annika Allard, G. Wadell, Mária Benkö

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

A primer pair originally designed for the universal detection of human adenovirus (HAV) serotypes of all subgenera was modified then tested and found feasible for the detection of different bovine, ovine, and porcine adenovirus (BAV, OAV, and PAV, respectively) serotypes. Apparently, in the examined viruses, parts of the DNA sequence coding for the basal part of the hexon protein are conserved enough for being applicable in polymerase chain reaction (PCR) as primers. Positive amplification could be obtained even from the so-called subgroup 2 BAVs, which viruses do not cross react with HAVs or subgroup 1 BAVs in Southern hybridisation.

Original languageEnglish
Pages (from-to)243-251
Number of pages9
JournalActa veterinaria Hungarica
Volume44
Issue number2
Publication statusPublished - Dec 1 1996

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Keywords

  • Adenovirus
  • Bovine
  • Hexon gene
  • Ovine
  • PCR
  • Porcine
  • Primer

ASJC Scopus subject areas

  • veterinary(all)

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