A hepatitis-C ribonukleinsav kimutatása polimeráz láncreakcióval.

Translated title of the contribution: Detection of hepatitis C viral RNA by polymerase chain reaction

A. Falus, E. Nemesánszky, A. Hajnal

Research output: Contribution to journalArticle

Abstract

The authors describe the development and the application of polymerase chain reaction (PCR) in the detection of hepatitis C virus. Based on the sequence data available in the literature, a conservative motif at the 5' end of the virus genome has been selected for amplification. Using the flanking synthetic oligonucleotides the specificity of the highly sensitive RNA-PCR assay was proved by serologically positive and negative serum samples. HCV-RNA-PCR studies were applied to start interferon treatment in patients from National Institute of Rheumatology and Physiotherapy (Budapest) with anti-HCV positive serum samples and this method was used to evaluate the efficacy of interferon therapy, as well.

Original languageHungarian
Pages (from-to)339-341
Number of pages3
JournalOrvosi hetilap
Volume136
Issue number7
Publication statusPublished - Feb 12 1995

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ASJC Scopus subject areas

  • Medicine(all)

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