Detection of equine herpesvirus-1 in the fetal membranes of aborted equine fetuses by immunohistochemical and in-situ hybridization techniques

L. Szeredi, H. Aupperle, K. Steiger

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Formalin-fixed, paraffin wax-embedded fetal membranes from 76 cases of equine abortion were examined immunohistochemically for equine herpesvirus (EHV)-l antigen. Of the 76 cases, 11 had been proved EHV-1-positive by diagnostic methods applied to the aborted fetuses (viral isolation in tissue culture, or immunohistochemical examination, or both). Of the 11 fetal membranes from the virus-positive animals, five gave positive results on immunohistochemical examination, and three on in-situ hybridization; the positive signals were detected in trophoblastic cells and occasionally in monocytes and endothelial cells. The distribution of virus appeared to be related to areas of (1) vacuolar degeneration and desquamation of chorionic epithelium, (2) mild lympho-histiocytic vasculitis and placentitis, and (3) increased metabolic activity of mesenchymal cells in the villi of the fetal membranes. This is the first report of EHV-1 antigen and nucleic acid detection in the trophoblasts of fetal membranes from spontaneous cases of equine abortion.

Original languageEnglish
Pages (from-to)147-153
Number of pages7
JournalJournal of Comparative Pathology
Volume129
Issue number2-3
DOIs
Publication statusPublished - Jan 1 2003

Keywords

  • Abotion
  • EHV-1
  • Equine herpesvirus-1
  • Fetal membranes
  • Herpesvirus
  • Horse
  • Viral infection

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • veterinary(all)

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