Detection of drug resistance in human ovarian carcinoma

M. Volm, T. Efferth, M. Bak, J. Mattern

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Drug-resistant cancer cells with the multidrug-resistance phenotype show overexpression of P-glycoprotein, and we therefore tested carcinoma tissue from five patients with stage III or IV ovarian cancer for P-glycoprotein using 265/F4 and C 219 monoclonal antibodies, prepared against membrane glycoproteins in colchicine-resistant CHO cells. Using immunofluorescence and immunoblotting techniques, one of the tumors showed a positive reaction. Using the pcDR 1.5 clone we found that the same cancer tissue had elevated expression of the genes responsible for multidrug resistance. The demonstration of elevated P-glycoprotein in ovarian carcinomas indicates that P-glycoprotein overexpression is not limited to experimental tumor models.

Original languageEnglish
Pages (from-to)123-128
Number of pages6
JournalArchives of Gynecology and Obstetrics
Volume244
Issue number2
DOIs
Publication statusPublished - Jun 1989

Fingerprint

P-Glycoprotein
Drug Resistance
Carcinoma
Neoplasms
MDR Genes
CHO Cells
Membrane Glycoproteins
Colchicine
Multiple Drug Resistance
Immunoblotting
Ovarian Neoplasms
Fluorescent Antibody Technique
Theoretical Models
Clone Cells
Monoclonal Antibodies
Phenotype
Pharmaceutical Preparations

Keywords

  • Human ovarian carcinoma
  • Monoclonal antibodies
  • Multidrug resistance
  • P-glycoprotein

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Detection of drug resistance in human ovarian carcinoma. / Volm, M.; Efferth, T.; Bak, M.; Mattern, J.

In: Archives of Gynecology and Obstetrics, Vol. 244, No. 2, 06.1989, p. 123-128.

Research output: Contribution to journalArticle

Volm, M. ; Efferth, T. ; Bak, M. ; Mattern, J. / Detection of drug resistance in human ovarian carcinoma. In: Archives of Gynecology and Obstetrics. 1989 ; Vol. 244, No. 2. pp. 123-128.
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