Detection of drug resistance in human ovarian carcinoma

M. Volm, T. Efferth, M. Bak, J. Mattern

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5 Citations (Scopus)

Abstract

Drug-resistant cancer cells with the multidrug-resistance phenotype show overexpression of P-glycoprotein, and we therefore tested carcinoma tissue from five patients with stage III or IV ovarian cancer for P-glycoprotein using 265/F4 and C 219 monoclonal antibodies, prepared against membrane glycoproteins in colchicine-resistant CHO cells. Using immunofluorescence and immunoblotting techniques, one of the tumors showed a positive reaction. Using the pcDR 1.5 clone we found that the same cancer tissue had elevated expression of the genes responsible for multidrug resistance. The demonstration of elevated P-glycoprotein in ovarian carcinomas indicates that P-glycoprotein overexpression is not limited to experimental tumor models.

Original languageEnglish
Pages (from-to)123-128
Number of pages6
JournalArchives of Gynecology and Obstetrics
Volume244
Issue number2
DOIs
Publication statusPublished - Jun 1 1989

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Keywords

  • Human ovarian carcinoma
  • Monoclonal antibodies
  • Multidrug resistance
  • P-glycoprotein

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

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