Detection of drug resistance and P-glycoprotein in human renal cell carcinomas

M. Bak, T. Efferth, G. Mickisch, J. Mattern, M. Volm

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Expression of the multidrug-resistance gene product P-glycoprotein (P-170) was screened in 21 untreated human renal cell carcinomas using monoclonal antibodies (265/F4, C219) and immunoperoxidase staining. The inherent drug resistance of the same samples against doxorubicin was established by a short-term chemo-resistance test in order to investigate the association between the expression of P-170 and intrinsic drug resistance in kidney cancers. P-glycoprotein could be demonstrated in 10 cases. Using the short-term test for predicting resistance, 17 resistant and 4 sensitive cancers were found. In vitro 10 of 17 resistant tumors revealed an increase of P-glycoprotein. On the other hand, in the sensitive tumor in vitro, an expression of P-glycoprotein could not be demonstrated. This investigation reveals that intrinsic drug resistance exists in many renal cell carcinomas and it is associated at least in part with increased expression of P-glycoprotein. The immunohistochemical results suggest that the presence of the P-glycoprotein may be useful as a marker for screening the multidrug-resistant phenotype in renal cell carcinomas and as an indicator of the therapeutic efficacy of multidrug-resistant kidney cancers.

Original languageEnglish
Pages (from-to)72-75
Number of pages4
JournalEuropean Urology
Volume17
Issue number1
Publication statusPublished - 1990

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P-Glycoprotein
Renal Cell Carcinoma
Drug Resistance
Kidney Neoplasms
MDR Genes
Neoplasms
Doxorubicin
Monoclonal Antibodies
Staining and Labeling
Phenotype

Keywords

  • glycoprotein P-170
  • in vitro test
  • monoclonal antibody
  • multidrug resistance
  • renal cell carcinoma

ASJC Scopus subject areas

  • Urology

Cite this

Bak, M., Efferth, T., Mickisch, G., Mattern, J., & Volm, M. (1990). Detection of drug resistance and P-glycoprotein in human renal cell carcinomas. European Urology, 17(1), 72-75.

Detection of drug resistance and P-glycoprotein in human renal cell carcinomas. / Bak, M.; Efferth, T.; Mickisch, G.; Mattern, J.; Volm, M.

In: European Urology, Vol. 17, No. 1, 1990, p. 72-75.

Research output: Contribution to journalArticle

Bak, M, Efferth, T, Mickisch, G, Mattern, J & Volm, M 1990, 'Detection of drug resistance and P-glycoprotein in human renal cell carcinomas', European Urology, vol. 17, no. 1, pp. 72-75.
Bak, M. ; Efferth, T. ; Mickisch, G. ; Mattern, J. ; Volm, M. / Detection of drug resistance and P-glycoprotein in human renal cell carcinomas. In: European Urology. 1990 ; Vol. 17, No. 1. pp. 72-75.
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