Detection of CCND1 locus amplification by fluorescence in situ hybridization

M. Balázs, Viktória Koroknai, István Szász, Szilvia Ecsedi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

It is well known that chromosomal aberrations of tumors are associated with the initiation and progression of malignancy. Fluorescence in situ hybridization (FISH) is a powerful, rapid method to detect chromosome copy number and structural alterations in tissue sections, chromosome, or interphase cellular preparations via hybridization of complementary probe sequences. The technique is based on the complementary nature of DNA double strands, which allows fluorescently labeled DNA probes to be used as probes to label the complementary sequences of target cells, chromosomes, and tissues. FISH technique has many applications, including basic gene mapping, used in pathological diagnosis to detect chromosome and gene copy number aberrations, translocations, microdeletions, and duplications. For the recognition of gene amplifications and deletions, locus-specific probes that are collections of one or a few cloned DNA sequences are routinely used. Multiplex-FISH (M-FISH) technique visualizes all chromosomes with different colors using spectrally distinct fluorophores for each chromosome in one experiment to detect numerical and structural alterations of chromosomes obtained from tumor cells. Recently many of the gene-specific probes are commercially available.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages85-100
Number of pages16
DOIs
Publication statusPublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1726
ISSN (Print)1064-3745

Fingerprint

Fluorescence In Situ Hybridization
Chromosomes
Neoplasms
Gene Dosage
Gene Amplification
Chromosome Mapping
Interphase
DNA Probes
Gene Deletion
Chromosome Aberrations
Complementary DNA
Color
Genes

Keywords

  • CCND1 copy number alteration
  • DNA probes
  • Fluorescence in situ hybridization
  • Gene amplification in interphase cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Balázs, M., Koroknai, V., Szász, I., & Ecsedi, S. (2018). Detection of CCND1 locus amplification by fluorescence in situ hybridization. In Methods in Molecular Biology (pp. 85-100). (Methods in Molecular Biology; Vol. 1726). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7565-5_9

Detection of CCND1 locus amplification by fluorescence in situ hybridization. / Balázs, M.; Koroknai, Viktória; Szász, István; Ecsedi, Szilvia.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 85-100 (Methods in Molecular Biology; Vol. 1726).

Research output: Chapter in Book/Report/Conference proceedingChapter

Balázs, M, Koroknai, V, Szász, I & Ecsedi, S 2018, Detection of CCND1 locus amplification by fluorescence in situ hybridization. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1726, Humana Press Inc., pp. 85-100. https://doi.org/10.1007/978-1-4939-7565-5_9
Balázs M, Koroknai V, Szász I, Ecsedi S. Detection of CCND1 locus amplification by fluorescence in situ hybridization. In Methods in Molecular Biology. Humana Press Inc. 2018. p. 85-100. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7565-5_9
Balázs, M. ; Koroknai, Viktória ; Szász, István ; Ecsedi, Szilvia. / Detection of CCND1 locus amplification by fluorescence in situ hybridization. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 85-100 (Methods in Molecular Biology).
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