Detection of bullous pemphigoid antibodies by means of synthetic peptides as antigenic epitopes

S. Husz, Mária Kiss, Ilona Marczinovits, G. K. Tóth, Katalin Molnár, J. Molnár, A. Dobozy

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Abstract

In an earlier study, the circulating autoantibodies in patients with bullous pemphigoid (BP) were demonstrated in higher frequencies by means of an immunoblot technique then via indirect immunofluorescence. In the present work with the help of Peptide Structure software, two matched antigenic epitopes were chosen and synthesized. The sera of 34 patients with BP were investigated in parallel by an immunoblot technique using a human epidermal extract, and by an ELISA technique with synthetic peptides. The sera of 16 healthy persons and 10 patients with other bullous diseases served as controls. Among the 34 patients with BP, 23 sera proved positive for at least one synthetic peptide. Positive reactions with the major BP antigen (230 kD) were found in 22 patients by immunoblot technique and in 16 patients by the ELISA technique with the synthetic peptide, whereas with the minor BP antigen (180 kD), positive reactions were found in 9 patients by the immunoblot technique and 18 patients by the ELISA technique using the synthetic antigenic epitope of the minor BP antigen. All control sera were negative for both antigens in ELISA investigations. In 3 patients with pemphigus vulgaris, characteristic bands against pemphigus vulgaris antigen were identified by means of the immunoblot technique, but all other cases were negative. It is suggested that the development of this technique may lead to an opportunity for the rapid and simple diagnosis of BP.

Original languageEnglish
Pages (from-to)339-343
Number of pages5
JournalActa microbiologica et immunologica Hungarica
Volume43
Issue number4
Publication statusPublished - Dec 1 1996

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

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