Detection of bovine herpesvirus 4 DNA in aborted bovie fetuses

Zoltán Deim, L. Szeredi, L. Egyed

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The presence of Bovine herpesvirus 4 (BoHV-4) was investigated by several methods in 24 aborted bovine fetuses. Polymerase chain reaction (PCR) and in situ DNA hybridization proved the presence of BoHV-4 DNA in 7 (29%) of the fetuses. The BoHV-4 genome was detected in the cytoplasm of splenic lymphocytes and monocytes, and sometimes in renal tubular epithelial cells or hepatic Kupffer cells, in all 7 PCR-positive fetuses. However, BoHV-4-specific monoclonal antibody failed to detect viral antigen in the formalin-fixed, paraffin-embedded tissue samples. No bacterial pathogens were found in the tissues of the BoHV-4-positive fetuses. Fungi were detected in 1 sample, and antibody to bovine viral diarrhea virus was detected in another. These results indicate that BoHV-4 could play a role in reproductive disorders of cattle, including abortion.

Original languageEnglish
Pages (from-to)226-229
Number of pages4
JournalCanadian Journal of Veterinary Research
Volume71
Issue number3
Publication statusPublished - Jul 2007

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Bovine Herpesvirus 4
Bovine herpesvirus 4
Aborted Fetus
fetus
DNA
Fetus
polymerase chain reaction
Bovine Viral Diarrhea Viruses
Kupffer cells
Polymerase Chain Reaction
abortion (animals)
reproductive disorders
Kupffer Cells
Viral Antigens
viral antigens
cattle
Bovine viral diarrhea virus
kidney cells
nucleic acid hybridization
formalin

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Detection of bovine herpesvirus 4 DNA in aborted bovie fetuses. / Deim, Zoltán; Szeredi, L.; Egyed, L.

In: Canadian Journal of Veterinary Research, Vol. 71, No. 3, 07.2007, p. 226-229.

Research output: Contribution to journalArticle

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