Detection of bovine gammaherpesviruses by a nested duplex PCR

Katalin Fábián, L. Egyed

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The two bovine gammaherpesviruses (Alcelaphine herpesvirus 1, AlHV-1; Bovine herpesvirus 4, BoHV-4) are distributed worldwide in cattle populations. Since the animals are frequently infected latently with no or low seropositivity, a DNA based diagnostic method would be useful for surveys and detection of these viruses. In the present study a nested duplex PCR was established for the sensitive and specific simultaneous detection of both viruses. The primers were designed for the gene of the major capsid protein (ORF25). The assay did not amplify the capsid gene sequence of 10 related bovine herpesviruses and other gammaherpesviruses. The test was able to detect 1pfu (plaque forming unit) of AlHV-1 and BoHV-4. Among 146 clinical samples (lymph nodes and peripheral blood leukocytes, PBLs) 65 (44.52%) were found to be positives for AlHV-1 and 84 (57.53%) for BoHV-4. This nested duplex PCR assay could serve as a useful diagnostic method for rapid, sensitive, specific simultaneous detection of the two bovine gammaherpesviruses.

Original languageEnglish
Pages (from-to)93-98
Number of pages6
JournalJournal of Virological Methods
Volume115
Issue number1
DOIs
Publication statusPublished - Jan 2004

Fingerprint

Polymerase Chain Reaction
Herpesviridae
Bovine Herpesvirus 4
Viruses
Capsid
Capsid Proteins
Genes
Leukocytes
Lymph Nodes
DNA
Population
Surveys and Questionnaires

Keywords

  • Alcelaphine herpesvirus 1
  • Bovine herpesvirus 4
  • Nested duplex PCR

ASJC Scopus subject areas

  • Virology

Cite this

Detection of bovine gammaherpesviruses by a nested duplex PCR. / Fábián, Katalin; Egyed, L.

In: Journal of Virological Methods, Vol. 115, No. 1, 01.2004, p. 93-98.

Research output: Contribution to journalArticle

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