Detection of borrelia burgdorferi sensu lato in lizards and their ticks from Hungary

G. Földvári, Krisztina Rigó, Viktória Majláthová, Igor Majláth, R. Farkas, Branislav Pet'Ko

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

To investigate the involvement of lizard species in the natural cycle of Borrelia burgdorferi sensu lato (s.l.) in Hungary, a total of 186 reptiles belonging to three species-126 green lizards (Lacerta viridis), 40 Balkan wall lizards (Podarcis taurica), and 20 sand lizards (Lacerta agilis)-were captured in 2007 and 2008. All ticks removed from the lizards were Ixodes ricinus, either larvae (324/472; 68.6%) or nymphs (148/472; 31.4%). More than half (66/126; 52.4%) of L. viridis individuals were infested, and the prevalence of tick infestation on both the other two species was 35% each. All 472 I. ricinus ticks and tissue samples collected from 134 collar scales and 62 toe clips of lizards were further analyzed for the presence of B. burgdorferi s.l. with polymerase chain reaction. The amplification of B. burgdorferi s.l. DNA was successful in 8% (n=92) of L. viridis, 9% (n=32) of P. taurica, and 10% (n=10) of L. agilis tissue samples. Restriction fragment length polymorphism genotyping identified the species Borrelia lusitaniae in all tested lizard samples. Prevalence of B. burgdorferi s.l. in ticks collected from L. viridis, P. taurica, and L. agilis was 8%, 2%, and 0%, respectively. Most of the infected ticks carried B. lusitaniae (74% of genotyped positives); however, Borrelia afzelii (5%) and B. burgdorferi sensu stricto (21%) were detected in ticks removed from green lizards and Balkan wall lizards, respectively. We conclude that lizards, particularly L. viridis, can be important hosts for I. ricinus larvae and nymphs; thus, they can be regarded as reservoirs of these important pathogen vectors. The role of green lizards has been confirmed, and the implication of Balkan wall lizards is suggested in the natural cycle of B. lusitaniae at our study site.

Original languageEnglish
Pages (from-to)331-336
Number of pages6
JournalVector-Borne and Zoonotic Diseases
Volume9
Issue number3
DOIs
Publication statusPublished - Jun 1 2009

Fingerprint

Borrelia burgdorferi Group
Lizards
Hungary
Ticks
Balkan Peninsula
Ixodes
Nymph
Larva
Tick Infestations
Borrelia
Borrelia burgdorferi
Reptiles
Toes
Surgical Instruments
Restriction Fragment Length Polymorphisms

Keywords

  • Borrelia burgdorferi sensu lato
  • Borrelia lusitaniae
  • Lacerta agilis
  • Lacerta viridis
  • Lizards
  • Podarcis taurica

ASJC Scopus subject areas

  • Infectious Diseases
  • Microbiology
  • Virology

Cite this

Detection of borrelia burgdorferi sensu lato in lizards and their ticks from Hungary. / Földvári, G.; Rigó, Krisztina; Majláthová, Viktória; Majláth, Igor; Farkas, R.; Pet'Ko, Branislav.

In: Vector-Borne and Zoonotic Diseases, Vol. 9, No. 3, 01.06.2009, p. 331-336.

Research output: Contribution to journalArticle

Földvári, G. ; Rigó, Krisztina ; Majláthová, Viktória ; Majláth, Igor ; Farkas, R. ; Pet'Ko, Branislav. / Detection of borrelia burgdorferi sensu lato in lizards and their ticks from Hungary. In: Vector-Borne and Zoonotic Diseases. 2009 ; Vol. 9, No. 3. pp. 331-336.
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