Detection of beet yellows virus in sugar beet leaves and roots by enzyme-linked immunosorbent assay (ELISA)

J. Polák, I. Barna-Vetró, Béa György, Věra Zindulková

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Using the enzyme-linked immunosorbent assay (ELISA) beet yellows virus (BYV) could be detected reliably in the leaves of sugar beet and Tetragonia expansa Pall. and in the roots of sugar beet. Specifio γ-globulin of BYV antiserum was coupled to horse radish peroxidase by periodate oxidation. Optimum dilutions of antigen (extract from infected leaves) were 1: 50 to 1: 200 for BYV detection in sugar beet and T. expansa leaves and 1: 2 to 1: 5 for detection in sugar beet roots. Extracts from beet roots are not to be purified by ultracentrifugation, however, by the described method virus can be demonstrated only in 80-90% of naturally infected sugar beet roots. The method is specific, no increase of extinction values was found in healthy or beet western yellows virus infected plants. Presence of virus can be demonstrated by visual as well as photometric evaluation. Results confirmed the suitability of peroxidase application for detection of plant viruses by ELISA.

Original languageEnglish
Pages (from-to)354-357
Number of pages4
JournalBiologia Plantarum
Volume22
Issue number5
DOIs
Publication statusPublished - Sep 1980

Fingerprint

Beet yellows virus
sugar beet
enzyme-linked immunosorbent assay
Tetragonia tetragonoides
leaves
peroxidase
Beet western yellows virus
horseradish
viruses
ultracentrifugation
plant viruses
extracts
beets
globulins
antiserum
extinction
oxidation
antigens
methodology

Keywords

  • immunoenzymatic reaction
  • leaves and roots
  • serological method
  • sugar beet

ASJC Scopus subject areas

  • Plant Science

Cite this

Detection of beet yellows virus in sugar beet leaves and roots by enzyme-linked immunosorbent assay (ELISA). / Polák, J.; Barna-Vetró, I.; György, Béa; Zindulková, Věra.

In: Biologia Plantarum, Vol. 22, No. 5, 09.1980, p. 354-357.

Research output: Contribution to journalArticle

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