Detection of Agrobacterium vitis by polymerase chain reaction in grapevine bleeding sap after isolation on a semiselective medium

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Abstract

DNA samples prepared from 15 Agrobacterium vitis and one A. tumefaciens strains were tested in polymerase chain reaction with 4 primer pairs. The pTiC58 virC specific primers did not detect any A. vitis, while the polygalacturonase specific primers resulted in positive reactions with all grapevine strains. Octopine and nopaline strains could be distinguished from vitopine strains by virE2 specific primers. Like for other bacterial species Triton X-100 with sodium-azide strongly increased the sensitivity of detection of A. vitis. Fourty-six colonies were isolated on a tartrate medium from grapevine bleeding sap collected from galled and symptomless Vitis vinifera cv. Cabernet Sauvignon and Riesling plants. Fourteen of them were identified by PCR as A. vitis, 11 of which proved to be pathogenic. Analysis of bleeding sap for A. vitis may become a useful diagnostic method for selection of healthy plants.

Original languageEnglish
Pages (from-to)37-42
Number of pages6
JournalVitis - Journal of Grapevine Research
Volume41
Issue number1
Publication statusPublished - 2002

Fingerprint

Rhizobium vitis
Agrobacterium
Vitis
sap
polymerase chain reaction
Hemorrhage
Polymerase Chain Reaction
octopine
nopaline
sodium azide
Polygalacturonase
polygalacturonase
Sodium Azide
Agrobacterium radiobacter
Vitis vinifera
diagnostic techniques
Octoxynol
DNA

Keywords

  • Agrobacterium vitis
  • Bleeding sap
  • Crown gall
  • Polymerase chain reaction
  • Sodium-azide

ASJC Scopus subject areas

  • Horticulture

Cite this

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abstract = "DNA samples prepared from 15 Agrobacterium vitis and one A. tumefaciens strains were tested in polymerase chain reaction with 4 primer pairs. The pTiC58 virC specific primers did not detect any A. vitis, while the polygalacturonase specific primers resulted in positive reactions with all grapevine strains. Octopine and nopaline strains could be distinguished from vitopine strains by virE2 specific primers. Like for other bacterial species Triton X-100 with sodium-azide strongly increased the sensitivity of detection of A. vitis. Fourty-six colonies were isolated on a tartrate medium from grapevine bleeding sap collected from galled and symptomless Vitis vinifera cv. Cabernet Sauvignon and Riesling plants. Fourteen of them were identified by PCR as A. vitis, 11 of which proved to be pathogenic. Analysis of bleeding sap for A. vitis may become a useful diagnostic method for selection of healthy plants.",
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T1 - Detection of Agrobacterium vitis by polymerase chain reaction in grapevine bleeding sap after isolation on a semiselective medium

AU - Szegedi, E.

AU - Bottka, S.

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N2 - DNA samples prepared from 15 Agrobacterium vitis and one A. tumefaciens strains were tested in polymerase chain reaction with 4 primer pairs. The pTiC58 virC specific primers did not detect any A. vitis, while the polygalacturonase specific primers resulted in positive reactions with all grapevine strains. Octopine and nopaline strains could be distinguished from vitopine strains by virE2 specific primers. Like for other bacterial species Triton X-100 with sodium-azide strongly increased the sensitivity of detection of A. vitis. Fourty-six colonies were isolated on a tartrate medium from grapevine bleeding sap collected from galled and symptomless Vitis vinifera cv. Cabernet Sauvignon and Riesling plants. Fourteen of them were identified by PCR as A. vitis, 11 of which proved to be pathogenic. Analysis of bleeding sap for A. vitis may become a useful diagnostic method for selection of healthy plants.

AB - DNA samples prepared from 15 Agrobacterium vitis and one A. tumefaciens strains were tested in polymerase chain reaction with 4 primer pairs. The pTiC58 virC specific primers did not detect any A. vitis, while the polygalacturonase specific primers resulted in positive reactions with all grapevine strains. Octopine and nopaline strains could be distinguished from vitopine strains by virE2 specific primers. Like for other bacterial species Triton X-100 with sodium-azide strongly increased the sensitivity of detection of A. vitis. Fourty-six colonies were isolated on a tartrate medium from grapevine bleeding sap collected from galled and symptomless Vitis vinifera cv. Cabernet Sauvignon and Riesling plants. Fourteen of them were identified by PCR as A. vitis, 11 of which proved to be pathogenic. Analysis of bleeding sap for A. vitis may become a useful diagnostic method for selection of healthy plants.

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