Detection of activation antigens on chronic lymphocytic leukaemia cells

K. Pálóczi, Éva Pócsik, R. Mihalik, Miklós Benczur, J. Demeter, Vera Solti, G. Petrányi, S. Hollán

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The peripheral blood mononuclear cells of patients with chronic lymphocytic leukaemia were characterized by the presence of a variety of cell surface differentiation antigens. The cells of 20 patients were found to be of B-cell phenotype when studied with antibodies directed against CD19, CD20, HLA-DR and sIg. Furthermore, a significant percentage of the cells gave a positive reaction with the monoclonal antibody to CD5. On the other hand, the CLL-cells did not express the CD21 antigen (C3d receptor, EBV receptor). We studied in parallel the presence of various activation antigens using 19 monoclonal antibodies grouped into 7 clusters (CD25, CD30, CD40, CD69, CD70, CD39, CD71). A significantly higher percentage of the CLL cells expressed activation antigens than lymphocytes from healthy controls. The percentage of CD3/HLA + DR + cells, compared to the healthy control lymphocytes was not increased in the CLL patients, and the activated cells in CLL were found to have characteristics of B-cells. Based on these results, we suggest that the CLL cells, like the cells in Hodgkin's disease and T-cell lymphoma, are not resting, but activated B-cells or the neoplastic abberrants of activated cells.

Original languageEnglish
Pages (from-to)31-36
Number of pages6
JournalLeukemia and Lymphoma
Volume3
Issue number1
DOIs
Publication statusPublished - 1990

Fingerprint

B-Cell Chronic Lymphocytic Leukemia
Antigens
Complement 3d Receptors
B-Lymphocytes
HLA-DR Antigens
Monoclonal Antibodies
Antigen Receptors
T-Cell Lymphoma
Differentiation Antigens
Surface Antigens
Lymphocyte Activation
Human Herpesvirus 4
Hodgkin Disease
Cell Differentiation
Blood Cells
Lymphocytes
Phenotype
Antibodies

Keywords

  • Activation antigens
  • CLL

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Detection of activation antigens on chronic lymphocytic leukaemia cells. / Pálóczi, K.; Pócsik, Éva; Mihalik, R.; Benczur, Miklós; Demeter, J.; Solti, Vera; Petrányi, G.; Hollán, S.

In: Leukemia and Lymphoma, Vol. 3, No. 1, 1990, p. 31-36.

Research output: Contribution to journalArticle

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