Detection of a putative novel adenovirus by PCR amplification, sequencing and phylogenetic characterisation of two gene fragments from formalin-fixed paraffin-embedded tissues of a cat diagnosed with disseminated adenovirus disease

Béla Lakatos, Ákos Hornyák, Zoltán Demeter, Petra Forgách, Frances Kennedy, Miklós Rusvai

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Adenoviral nucleic acid was detected by polymerase chain reaction (PCR) in formalin-fixed paraffin-embedded tissue samples of a cat that had suffered from disseminated adenovirus infection. The identity of the amplified products from the hexon and DNA-dependent DNA polymerase genes was confirmed by DNA sequencing. The sequences were clearly distinguishable from corresponding hexon and polymerase sequences of other mastadenoviruses, including human adenoviruses. These results suggest the possible existence of a distinct feline adenovirus.

Original languageEnglish
Pages (from-to)574-584
Number of pages11
JournalActa veterinaria Hungarica
Volume65
Issue number4
DOIs
Publication statusPublished - Dec 2017

Keywords

  • Adenovirus
  • Cat
  • DNA polymerase
  • FFPE
  • Hexon
  • PCR

ASJC Scopus subject areas

  • veterinary(all)

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