Detection and typing of 46 genital human papillomaviruses by the L1F/L1R primer system based multiplex PCR and hybridization

Csaba Jeney, Tibor Takács, Attila Sebe, Z. Schaff

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

There are several genital HPV DNA detection systems described, however most of them present different disadvantages regarding the number of amplified and detected types, sensitivity, type specificity. A new PCR and hybridization based detection method was developed for sensitive and balanced amplification and specific typing of HPV DNA from clinical samples. The technique amplifies and detects 46 HPV types: 2a, 3, 6, 7, 10, 11, 13, 16, 18, 26, 27, 28, 29, 30, 31, 33, 34, 35, 39, 40, 42, 43, 44/55, 45, 51, 52, 53, 54, 56, 57, 58, 59, 61, 66, 67, 68, 70, 72, 73(MM9), 74, 82(MM4), 84(MM8), 89, 90, 91. Key elements of the L1F/L1R PCR and hybridization system are: the special selection of the amplified region, a novel and optimized amplification primer set, circumspectly designed general and type-specific oligonucleotide probes. Detection following a multiplex PCR is based on solid phase hybridization in microtiter plate format using general and type-specific probes at medium stringency, which makes the detection robust in case of small sequence variations. The assay is highly reproducible and suitable for automation. The method was compared to Hybrid Capture II test, and after clarifying conflicting results, the comparison showed an excellent agreement (96.2%).

Original languageEnglish
Pages (from-to)32-42
Number of pages11
JournalJournal of Virological Methods
Volume140
Issue number1-2
DOIs
Publication statusPublished - Mar 2007

Fingerprint

Multiplex Polymerase Chain Reaction
Polymerase Chain Reaction
DNA Fingerprinting
Oligonucleotide Probes
Automation
Sensitivity and Specificity
DNA

Keywords

  • HPV detection
  • HPV typing
  • Hybridization
  • Multiplex PCR

ASJC Scopus subject areas

  • Virology

Cite this

Detection and typing of 46 genital human papillomaviruses by the L1F/L1R primer system based multiplex PCR and hybridization. / Jeney, Csaba; Takács, Tibor; Sebe, Attila; Schaff, Z.

In: Journal of Virological Methods, Vol. 140, No. 1-2, 03.2007, p. 32-42.

Research output: Contribution to journalArticle

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