Detection and relocation of rare events: A comparative study using the laser scanning cytometer and the Metafer/RCDetect microscope scanning system

Simone Hennerbichler, Gabor Méhes, Rudolf Schmied, Reinhold Wintersteiger, Gottfried Dohr, Peter Ambros, Peter Sedlmayr

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6 Citations (Scopus)

Abstract

We compared instrumental analysis of enriched cord blood nucleated red blood cells (CB-NRBC) out of in vitro contamination preparations of dilutions of minute volumes of male cord blood into peripheral blood from nonpregnant women. This was done using the laser scanning cytometer (LSC) and the Metafer/RCDetect microscope scanning system, both allowing for relocation of positive cells defined on the basis of fluorescence parameters. Both instruments were efficient in performing scanning and relocation; a difference in the recovery of CB-NRBC was not significant and can be explained by the method of preparation used.

Original languageEnglish
Pages (from-to)109-115
Number of pages7
JournalJournal of biochemical and biophysical methods
Volume53
Issue number1-3
DOIs
Publication statusPublished - Oct 1 2002

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Keywords

  • Laser scanning cytometry
  • Magnetic cell sorting
  • Metafer/RCDetect
  • Rare cells

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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