Detection and proteomic characterization of extracellular vesicles in human pancreatic juice

Xabier Osteikoetxea, Márton Benke, Marta Rodriguez, Krisztina Pálóczi, Barbara W. Sódar, Zsuzsanna Szvicsek, Katalin Szabó-Taylor, Krisztina V. Vukman, A. Kittel, Zoltán Wiener, K. Vékey, László Harsányi, Ákos Szűcs, Lilla Turiák, E. Búzás

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Aims: The prognosis of patients with pancreatic cancer has remained virtually unchanged with a high mortality rate compared to other types of cancers. An earlier detection would provide a time window of opportunity for treatment and prevention of deaths. In the present study we investigated extracellular vesicle (EV)-associated potential biomarkers for pancreatic cancer by directly assessing EV size-based subpopulations in pancreatic juice samples of patients with chronic pancreatitis or pancreatic cancer. In addition, we also studied blood plasma and pancreatic cancer cell line-derived EVs. Methods: Comparative proteomic analysis was performed of 102 EV preparations from human pancreatic juices, blood, and pancreatic cancer cell lines Capan-1 and MIA PaCa-2. EV preparations were also characterized by electron microscopy, tunable resistive pulse sensing, and flow cytometry. Results: Here we describe the presence of EVs in human pancreatic juice samples. Pancreatic juice EV-associated proteins that we identified as possible candidate markers for pancreatic cancer included mucins, such as MUC1, MUC4, MUC5AC, MUC6 and MUC16, CFTR, and MDR1 proteins. These candidate biomarkers could also be detected by flow cytometry in EVs found in pancreatic juice and those secreted by pancreatic cancer cell lines. Conclusions: Together our data show that detection and characterization of EVs directly in pancreatic juice is feasible and may prove to be a valuable source of potential biomarkers of pancreatic cancer.

Original languageEnglish
Pages (from-to)37-43
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume499
Issue number1
DOIs
Publication statusPublished - Apr 30 2018

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Pancreatic Juice
Biomarkers
Pancreatic Neoplasms
Proteomics
Flow cytometry
Cells
Blood
Cystic Fibrosis Transmembrane Conductance Regulator
P-Glycoprotein
Mucins
Electron microscopy
Cell Line
Plasmas
Flow Cytometry
Extracellular Vesicles
Chronic Pancreatitis
Proteins
Electron Microscopy
Mortality

Keywords

  • Exosomes
  • Extracellular vesicles
  • Pancreas
  • Pancreatic cancer
  • Tumor markers

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Detection and proteomic characterization of extracellular vesicles in human pancreatic juice. / Osteikoetxea, Xabier; Benke, Márton; Rodriguez, Marta; Pálóczi, Krisztina; Sódar, Barbara W.; Szvicsek, Zsuzsanna; Szabó-Taylor, Katalin; Vukman, Krisztina V.; Kittel, A.; Wiener, Zoltán; Vékey, K.; Harsányi, László; Szűcs, Ákos; Turiák, Lilla; Búzás, E.

In: Biochemical and Biophysical Research Communications, Vol. 499, No. 1, 30.04.2018, p. 37-43.

Research output: Contribution to journalArticle

Osteikoetxea, X, Benke, M, Rodriguez, M, Pálóczi, K, Sódar, BW, Szvicsek, Z, Szabó-Taylor, K, Vukman, KV, Kittel, A, Wiener, Z, Vékey, K, Harsányi, L, Szűcs, Á, Turiák, L & Búzás, E 2018, 'Detection and proteomic characterization of extracellular vesicles in human pancreatic juice', Biochemical and Biophysical Research Communications, vol. 499, no. 1, pp. 37-43. https://doi.org/10.1016/j.bbrc.2018.03.107
Osteikoetxea, Xabier ; Benke, Márton ; Rodriguez, Marta ; Pálóczi, Krisztina ; Sódar, Barbara W. ; Szvicsek, Zsuzsanna ; Szabó-Taylor, Katalin ; Vukman, Krisztina V. ; Kittel, A. ; Wiener, Zoltán ; Vékey, K. ; Harsányi, László ; Szűcs, Ákos ; Turiák, Lilla ; Búzás, E. / Detection and proteomic characterization of extracellular vesicles in human pancreatic juice. In: Biochemical and Biophysical Research Communications. 2018 ; Vol. 499, No. 1. pp. 37-43.
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abstract = "Aims: The prognosis of patients with pancreatic cancer has remained virtually unchanged with a high mortality rate compared to other types of cancers. An earlier detection would provide a time window of opportunity for treatment and prevention of deaths. In the present study we investigated extracellular vesicle (EV)-associated potential biomarkers for pancreatic cancer by directly assessing EV size-based subpopulations in pancreatic juice samples of patients with chronic pancreatitis or pancreatic cancer. In addition, we also studied blood plasma and pancreatic cancer cell line-derived EVs. Methods: Comparative proteomic analysis was performed of 102 EV preparations from human pancreatic juices, blood, and pancreatic cancer cell lines Capan-1 and MIA PaCa-2. EV preparations were also characterized by electron microscopy, tunable resistive pulse sensing, and flow cytometry. Results: Here we describe the presence of EVs in human pancreatic juice samples. Pancreatic juice EV-associated proteins that we identified as possible candidate markers for pancreatic cancer included mucins, such as MUC1, MUC4, MUC5AC, MUC6 and MUC16, CFTR, and MDR1 proteins. These candidate biomarkers could also be detected by flow cytometry in EVs found in pancreatic juice and those secreted by pancreatic cancer cell lines. Conclusions: Together our data show that detection and characterization of EVs directly in pancreatic juice is feasible and may prove to be a valuable source of potential biomarkers of pancreatic cancer.",
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AU - Benke, Márton

AU - Rodriguez, Marta

AU - Pálóczi, Krisztina

AU - Sódar, Barbara W.

AU - Szvicsek, Zsuzsanna

AU - Szabó-Taylor, Katalin

AU - Vukman, Krisztina V.

AU - Kittel, A.

AU - Wiener, Zoltán

AU - Vékey, K.

AU - Harsányi, László

AU - Szűcs, Ákos

AU - Turiák, Lilla

AU - Búzás, E.

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N2 - Aims: The prognosis of patients with pancreatic cancer has remained virtually unchanged with a high mortality rate compared to other types of cancers. An earlier detection would provide a time window of opportunity for treatment and prevention of deaths. In the present study we investigated extracellular vesicle (EV)-associated potential biomarkers for pancreatic cancer by directly assessing EV size-based subpopulations in pancreatic juice samples of patients with chronic pancreatitis or pancreatic cancer. In addition, we also studied blood plasma and pancreatic cancer cell line-derived EVs. Methods: Comparative proteomic analysis was performed of 102 EV preparations from human pancreatic juices, blood, and pancreatic cancer cell lines Capan-1 and MIA PaCa-2. EV preparations were also characterized by electron microscopy, tunable resistive pulse sensing, and flow cytometry. Results: Here we describe the presence of EVs in human pancreatic juice samples. Pancreatic juice EV-associated proteins that we identified as possible candidate markers for pancreatic cancer included mucins, such as MUC1, MUC4, MUC5AC, MUC6 and MUC16, CFTR, and MDR1 proteins. These candidate biomarkers could also be detected by flow cytometry in EVs found in pancreatic juice and those secreted by pancreatic cancer cell lines. Conclusions: Together our data show that detection and characterization of EVs directly in pancreatic juice is feasible and may prove to be a valuable source of potential biomarkers of pancreatic cancer.

AB - Aims: The prognosis of patients with pancreatic cancer has remained virtually unchanged with a high mortality rate compared to other types of cancers. An earlier detection would provide a time window of opportunity for treatment and prevention of deaths. In the present study we investigated extracellular vesicle (EV)-associated potential biomarkers for pancreatic cancer by directly assessing EV size-based subpopulations in pancreatic juice samples of patients with chronic pancreatitis or pancreatic cancer. In addition, we also studied blood plasma and pancreatic cancer cell line-derived EVs. Methods: Comparative proteomic analysis was performed of 102 EV preparations from human pancreatic juices, blood, and pancreatic cancer cell lines Capan-1 and MIA PaCa-2. EV preparations were also characterized by electron microscopy, tunable resistive pulse sensing, and flow cytometry. Results: Here we describe the presence of EVs in human pancreatic juice samples. Pancreatic juice EV-associated proteins that we identified as possible candidate markers for pancreatic cancer included mucins, such as MUC1, MUC4, MUC5AC, MUC6 and MUC16, CFTR, and MDR1 proteins. These candidate biomarkers could also be detected by flow cytometry in EVs found in pancreatic juice and those secreted by pancreatic cancer cell lines. Conclusions: Together our data show that detection and characterization of EVs directly in pancreatic juice is feasible and may prove to be a valuable source of potential biomarkers of pancreatic cancer.

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