Detection and molecular characterization of porcine picobirnavirus in feces of domestic pigs from Kolkata, India

Balasubramanian Ganesh, Krisztián Bányai, Suman Kanungo, Deepika Sur, Yashpal Singh Malik, Nobumichi Kobayashi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Picobirnaviruses (PBVs) are small, non-enveloped, 35-41 nm virion with bisegmented double-stranded RNA genome. PBVs are widespread and were detected in feces of humans and a wide variety of animals. Domestic pig, one of the ubiquitous farm animal reported incessant association with a variety of viral zoonoses. The objective of our study is to find out the incidence of PBV infection in healthy domestic pigs. The study was conducted by collecting feces of healthy/asymptomatic pigs from a piggery located in an urban slum at Kolkata, India to detect PBV infections. All the 11 fecal samples were tested by polyacrylamide gel electrophoresis and reverse transcription-polymerase chain reaction assay. In this study, we report the first incidence of detection and molecular characterization of porcine PBV (BG-Por-2/2010 and BG-Por-7/2010) in feces of domestic pigs from India using the human PBV genogroup I specific primer pair: PicoB25(+) and PicoB43(-). Sequence comparison and phylogenetic analysis of partial RNA-dependent RNA polymerase gene of genome segment 2 revealed genetic relatedness to hitherto reported porcine, murine and human genogroup I PBVs from different geographical regions. This warrants a stringent global surveillance to study the potential zoonotic and emerging PBV infections.

Original languageEnglish
Pages (from-to)387-391
Number of pages5
JournalIndian Journal of Virology
Volume23
Issue number3
DOIs
Publication statusPublished - Dec 1 2012

    Fingerprint

Keywords

  • Domestic pigs
  • Genogroup I PBVs
  • Genomic diversity
  • Picobirnaviruses (PBVs)
  • Zoonoses

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this