Detection and molecular characterization of porcine picobirnavirus in feces of domestic pigs from Kolkata, India

Balasubramanian Ganesh, K. Bányai, Suman Kanungo, Deepika Sur, Yashpal Singh Malik, Nobumichi Kobayashi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Picobirnaviruses (PBVs) are small, non-enveloped, 35-41 nm virion with bisegmented double-stranded RNA genome. PBVs are widespread and were detected in feces of humans and a wide variety of animals. Domestic pig, one of the ubiquitous farm animal reported incessant association with a variety of viral zoonoses. The objective of our study is to find out the incidence of PBV infection in healthy domestic pigs. The study was conducted by collecting feces of healthy/asymptomatic pigs from a piggery located in an urban slum at Kolkata, India to detect PBV infections. All the 11 fecal samples were tested by polyacrylamide gel electrophoresis and reverse transcription-polymerase chain reaction assay. In this study, we report the first incidence of detection and molecular characterization of porcine PBV (BG-Por-2/2010 and BG-Por-7/2010) in feces of domestic pigs from India using the human PBV genogroup I specific primer pair: PicoB25(+) and PicoB43(-). Sequence comparison and phylogenetic analysis of partial RNA-dependent RNA polymerase gene of genome segment 2 revealed genetic relatedness to hitherto reported porcine, murine and human genogroup I PBVs from different geographical regions. This warrants a stringent global surveillance to study the potential zoonotic and emerging PBV infections.

Original languageEnglish
Pages (from-to)387-391
Number of pages5
JournalIndian Journal of Virology
Volume23
Issue number3
DOIs
Publication statusPublished - Dec 2012

Fingerprint

Picobirnavirus
Sus scrofa
Feces
India
Swine
Zoonoses
Infection
Genotype
Genome
Poverty Areas
RNA Replicase
Double-Stranded RNA
Incidence
Domestic Animals
Virion
Reverse Transcription
Polyacrylamide Gel Electrophoresis

Keywords

  • Domestic pigs
  • Genogroup I PBVs
  • Genomic diversity
  • Picobirnaviruses (PBVs)
  • Zoonoses

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Detection and molecular characterization of porcine picobirnavirus in feces of domestic pigs from Kolkata, India. / Ganesh, Balasubramanian; Bányai, K.; Kanungo, Suman; Sur, Deepika; Malik, Yashpal Singh; Kobayashi, Nobumichi.

In: Indian Journal of Virology, Vol. 23, No. 3, 12.2012, p. 387-391.

Research output: Contribution to journalArticle

Ganesh, Balasubramanian ; Bányai, K. ; Kanungo, Suman ; Sur, Deepika ; Malik, Yashpal Singh ; Kobayashi, Nobumichi. / Detection and molecular characterization of porcine picobirnavirus in feces of domestic pigs from Kolkata, India. In: Indian Journal of Virology. 2012 ; Vol. 23, No. 3. pp. 387-391.
@article{356dd3bf03494b76a0b4870f63059740,
title = "Detection and molecular characterization of porcine picobirnavirus in feces of domestic pigs from Kolkata, India",
abstract = "Picobirnaviruses (PBVs) are small, non-enveloped, 35-41 nm virion with bisegmented double-stranded RNA genome. PBVs are widespread and were detected in feces of humans and a wide variety of animals. Domestic pig, one of the ubiquitous farm animal reported incessant association with a variety of viral zoonoses. The objective of our study is to find out the incidence of PBV infection in healthy domestic pigs. The study was conducted by collecting feces of healthy/asymptomatic pigs from a piggery located in an urban slum at Kolkata, India to detect PBV infections. All the 11 fecal samples were tested by polyacrylamide gel electrophoresis and reverse transcription-polymerase chain reaction assay. In this study, we report the first incidence of detection and molecular characterization of porcine PBV (BG-Por-2/2010 and BG-Por-7/2010) in feces of domestic pigs from India using the human PBV genogroup I specific primer pair: PicoB25(+) and PicoB43(-). Sequence comparison and phylogenetic analysis of partial RNA-dependent RNA polymerase gene of genome segment 2 revealed genetic relatedness to hitherto reported porcine, murine and human genogroup I PBVs from different geographical regions. This warrants a stringent global surveillance to study the potential zoonotic and emerging PBV infections.",
keywords = "Domestic pigs, Genogroup I PBVs, Genomic diversity, Picobirnaviruses (PBVs), Zoonoses",
author = "Balasubramanian Ganesh and K. B{\'a}nyai and Suman Kanungo and Deepika Sur and Malik, {Yashpal Singh} and Nobumichi Kobayashi",
year = "2012",
month = "12",
doi = "10.1007/s13337-012-0106-z",
language = "English",
volume = "23",
pages = "387--391",
journal = "VirusDisease",
issn = "2347-3584",
publisher = "Springer India",
number = "3",

}

TY - JOUR

T1 - Detection and molecular characterization of porcine picobirnavirus in feces of domestic pigs from Kolkata, India

AU - Ganesh, Balasubramanian

AU - Bányai, K.

AU - Kanungo, Suman

AU - Sur, Deepika

AU - Malik, Yashpal Singh

AU - Kobayashi, Nobumichi

PY - 2012/12

Y1 - 2012/12

N2 - Picobirnaviruses (PBVs) are small, non-enveloped, 35-41 nm virion with bisegmented double-stranded RNA genome. PBVs are widespread and were detected in feces of humans and a wide variety of animals. Domestic pig, one of the ubiquitous farm animal reported incessant association with a variety of viral zoonoses. The objective of our study is to find out the incidence of PBV infection in healthy domestic pigs. The study was conducted by collecting feces of healthy/asymptomatic pigs from a piggery located in an urban slum at Kolkata, India to detect PBV infections. All the 11 fecal samples were tested by polyacrylamide gel electrophoresis and reverse transcription-polymerase chain reaction assay. In this study, we report the first incidence of detection and molecular characterization of porcine PBV (BG-Por-2/2010 and BG-Por-7/2010) in feces of domestic pigs from India using the human PBV genogroup I specific primer pair: PicoB25(+) and PicoB43(-). Sequence comparison and phylogenetic analysis of partial RNA-dependent RNA polymerase gene of genome segment 2 revealed genetic relatedness to hitherto reported porcine, murine and human genogroup I PBVs from different geographical regions. This warrants a stringent global surveillance to study the potential zoonotic and emerging PBV infections.

AB - Picobirnaviruses (PBVs) are small, non-enveloped, 35-41 nm virion with bisegmented double-stranded RNA genome. PBVs are widespread and were detected in feces of humans and a wide variety of animals. Domestic pig, one of the ubiquitous farm animal reported incessant association with a variety of viral zoonoses. The objective of our study is to find out the incidence of PBV infection in healthy domestic pigs. The study was conducted by collecting feces of healthy/asymptomatic pigs from a piggery located in an urban slum at Kolkata, India to detect PBV infections. All the 11 fecal samples were tested by polyacrylamide gel electrophoresis and reverse transcription-polymerase chain reaction assay. In this study, we report the first incidence of detection and molecular characterization of porcine PBV (BG-Por-2/2010 and BG-Por-7/2010) in feces of domestic pigs from India using the human PBV genogroup I specific primer pair: PicoB25(+) and PicoB43(-). Sequence comparison and phylogenetic analysis of partial RNA-dependent RNA polymerase gene of genome segment 2 revealed genetic relatedness to hitherto reported porcine, murine and human genogroup I PBVs from different geographical regions. This warrants a stringent global surveillance to study the potential zoonotic and emerging PBV infections.

KW - Domestic pigs

KW - Genogroup I PBVs

KW - Genomic diversity

KW - Picobirnaviruses (PBVs)

KW - Zoonoses

UR - http://www.scopus.com/inward/record.url?scp=84879554581&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84879554581&partnerID=8YFLogxK

U2 - 10.1007/s13337-012-0106-z

DO - 10.1007/s13337-012-0106-z

M3 - Article

AN - SCOPUS:84879554581

VL - 23

SP - 387

EP - 391

JO - VirusDisease

JF - VirusDisease

SN - 2347-3584

IS - 3

ER -