Detection and localization of two hydrogenases in Methylococcus capsulatus (Bath) and their potential role in methane metabolism

Tímea Hanczár, Robert Csáki, Levente Bodrossy, Colin J. Murrell, Kornél L. Kovács

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Methylococcus capsulatus (Bath) was shown to contain two distinct hydrogenases, a soluble hydrogenase and a membrane-bound hydrogenase. This is the first report of a membrane-bound hydrogenase in methanotrophs. Both enzymes were expressed apparently constitutively under normal growth conditions. The soluble hydrogenase was capable of reducing NAD+ with molecular hydrogen. The activities of both soluble and particulate methane monooxygenases could be driven by molecular hydrogen. This confirmed that molecular hydrogen could be used as a source of reducing power for methane oxidation. Hydrogen-driven methane monooxygenase activities tolerated elevated temperatures and moderate oxygen concentrations. The significance of these findings for biotechnological applications of methanotrophs is discussed.

Original languageEnglish
Pages (from-to)167-172
Number of pages6
JournalArchives of Microbiology
Volume177
Issue number2
DOIs
Publication statusPublished - Feb 23 2002

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Keywords

  • Hydrogen metabolism
  • Metalloenzyme
  • Methane metabolism
  • Methane monooxygenase
  • Methanotrophic bacteria
  • Methyloccoccus capsulatus (Bath)
  • NAD-reducing hydrogenase
  • Thermotolerance
  • [NiFe]hydrogenase

ASJC Scopus subject areas

  • Microbiology
  • Biochemistry
  • Molecular Biology
  • Genetics

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