Detection and localization of two hydrogenases in Methylococcus capsulatus (Bath) and their potential role in methane metabolism

Tímea Hanczár, Robert Csáki, Levente Bodrossy, Colin J. Murrell, Kornél L. Kovács

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24 Citations (Scopus)


Methylococcus capsulatus (Bath) was shown to contain two distinct hydrogenases, a soluble hydrogenase and a membrane-bound hydrogenase. This is the first report of a membrane-bound hydrogenase in methanotrophs. Both enzymes were expressed apparently constitutively under normal growth conditions. The soluble hydrogenase was capable of reducing NAD+ with molecular hydrogen. The activities of both soluble and particulate methane monooxygenases could be driven by molecular hydrogen. This confirmed that molecular hydrogen could be used as a source of reducing power for methane oxidation. Hydrogen-driven methane monooxygenase activities tolerated elevated temperatures and moderate oxygen concentrations. The significance of these findings for biotechnological applications of methanotrophs is discussed.

Original languageEnglish
Pages (from-to)167-172
Number of pages6
JournalArchives of Microbiology
Issue number2
Publication statusPublished - Feb 23 2002



  • Hydrogen metabolism
  • Metalloenzyme
  • Methane metabolism
  • Methane monooxygenase
  • Methanotrophic bacteria
  • Methyloccoccus capsulatus (Bath)
  • NAD-reducing hydrogenase
  • Thermotolerance
  • [NiFe]hydrogenase

ASJC Scopus subject areas

  • Microbiology
  • Biochemistry
  • Molecular Biology
  • Genetics

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