Detection and isolation of cell-derived microparticles are compromised by protein complexes resulting from shared biophysical parameters

Bence György, K. Módos, E. Pállinger, Krisztina Pálóczi, Mária Pásztói, Petra Misják, M. Deli, Áron Sipos, Anikó Szalai, István Voszka, Anna Polgár, Kálmán Tóth, M. Csete, György Nagy, Steffen Gay, A. Falus, A. Kittel, E. Búzás

Research output: Contribution to journalArticle

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Abstract

Numerous diseases, recently reported to associate with elevated microvesicle/ microparticle (MP) counts, have also long been known to be characterized by accelerated immune complex (IC) formation. The goal of this study was to investigate the potential overlap between parameters of protein complexes (eg, ICs or avidinbiotin complexes) and MPs, which might perturb detection and/or isolation of MPs. In this work, after comprehensive characterization of MPs by electron microscopy, atomic force microscopy, dynamic light-scattering analysis, and flow cytometry, for the first time, we drive attention to the fact that protein complexes, especially insoluble ICs, overlap in biophysical properties (size, light scattering, and sedimentation) with MPs. This, in turn, affects MP quantification by flow cytometry and purification by differential centrifugation, especially in diseases in which IC formation is common, including not only autoimmune diseases, but also hematologic disorders, infections, and cancer. These data may necessitate reevaluation of certain published data on patient-derived MPs and contribute to correct the clinical laboratory assessment of the presence and biologic functions of MPs in health and disease.

Original languageEnglish
JournalBlood
Volume117
Issue number4
DOIs
Publication statusPublished - Jan 27 2011

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Cell-Derived Microparticles
Antigen-Antibody Complex
Flow cytometry
Flow Cytometry
Proteins
Atomic Force Microscopy
Clinical laboratories
Centrifugation
Autoimmune Diseases
Electron Microscopy
Dynamic light scattering
Sedimentation
Light scattering
Electron microscopy
Purification
Light
Atomic force microscopy
Health
Infection
Neoplasms

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

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Detection and isolation of cell-derived microparticles are compromised by protein complexes resulting from shared biophysical parameters. / György, Bence; Módos, K.; Pállinger, E.; Pálóczi, Krisztina; Pásztói, Mária; Misják, Petra; Deli, M.; Sipos, Áron; Szalai, Anikó; Voszka, István; Polgár, Anna; Tóth, Kálmán; Csete, M.; Nagy, György; Gay, Steffen; Falus, A.; Kittel, A.; Búzás, E.

In: Blood, Vol. 117, No. 4, 27.01.2011.

Research output: Contribution to journalArticle

György, Bence ; Módos, K. ; Pállinger, E. ; Pálóczi, Krisztina ; Pásztói, Mária ; Misják, Petra ; Deli, M. ; Sipos, Áron ; Szalai, Anikó ; Voszka, István ; Polgár, Anna ; Tóth, Kálmán ; Csete, M. ; Nagy, György ; Gay, Steffen ; Falus, A. ; Kittel, A. ; Búzás, E. / Detection and isolation of cell-derived microparticles are compromised by protein complexes resulting from shared biophysical parameters. In: Blood. 2011 ; Vol. 117, No. 4.
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