Detection and identification of stolbur phytoplasma in Hungary by PCR and RFLP methods

O. Viczián, S. Süle, R. Gáborjányi

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8 Citations (Scopus)


Malformed and yellows-diseased plants of pepper, tomato, tobacco, carrot, parsley, celery, grape, rape, jimsonweed (Datura stramonium), dandelion (Taraxacum officinale) and bladder campion (Silene vulgaris) collected in Hungary were examined for phytoplasma infection. DNA was extracted from midribs by an MLO enrichment method and subjected to polymerase chain reaction (PCR) using universal (fP1/rP7) and specific primer pairs (fSTOL/rSOLS) directed to ribosomal sequences. Phytoplasmas detected by PCR were characterized using restriction fragment length polymorphism (RFLP) analysis of PCR amplified DNA. Phytoplasmas identified in all examined plants were typical stolbur phytoplasmas. RFLP analysis of the PCR amplified ribosomal DNA from all plant species revealed an uniform pattern that was identical to that of stolbur phytoplasma collected in Serbia from pepper. Special malformations were observed on several crops and weeds suggesting hormonal imbalance. Even the overwintering roots of carrot were infected that indicates that the disease overwinters not only in weeds but also in the roots of cultivated plants. Phytoplasma was transmitted from celery leaves to periwinkle plants with dodder (Cuscuta campestris). This is the first report detecting stolbur phytoplasma on dandelion (Taraxacum officinale) and bladder campion (Silene vulgaris).

Original languageEnglish
Pages (from-to)255-260
Number of pages6
JournalActa Phytopathologica et Entomologica Hungarica
Issue number3-4
Publication statusPublished - Dec 1 1998

ASJC Scopus subject areas

  • Plant Science
  • Insect Science

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