Detection and characterization of canine astroviruses

Vito Martella, Paschalina Moschidou, Eleonora Lorusso, Viviana Mari, Michele Camero, Annalucia Bellacicco, Michele Losurdo, Pierfrancesco Pinto, Costantina Desario, K. Bányai, Gabriella Elia, Nicola Decaro, Canio Buonavoglia

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Astroviruses (AstVs) have been identified only occasionally in dogs. A canine AstV, strain Bari/ 08/ITA, was detected from a pup with gastroenteric signs and the virus was isolated in cell culture and characterized molecularly. In the full-length capsid protein, the virus displayed genetic similarities (83.5% aa identity) to another canine AstV strain, although a high rate of variation occurred in the hypervariable domain, which is related to AstV antigenic specificity. Specific antibodies were detected in the convalescent dog, indicating seroconversion, and in 59% of a collection of dog serum samples. Using primers specific for canine AstV, designed to detect a conserved region of ORF1b, canine AstVs were detected in 24.5% of young pups with gastroenteritis, either alone or in mixed infections with other canine pathogens. In contrast, AstVs were detected in only 9.3% of asymptomatic pups. These findings indicate that canine AstVs are common in dogs and may suggest a possible role as canine enteric pathogens.

Original languageEnglish
Pages (from-to)1880-1887
Number of pages8
JournalJournal of General Virology
Volume92
Issue number8
DOIs
Publication statusPublished - Aug 2011

Fingerprint

Canidae
Dogs
Viruses
Gastroenteritis
Capsid Proteins
Coinfection
Epitopes
Cell Culture Techniques
Antibodies
Serum

ASJC Scopus subject areas

  • Virology

Cite this

Martella, V., Moschidou, P., Lorusso, E., Mari, V., Camero, M., Bellacicco, A., ... Buonavoglia, C. (2011). Detection and characterization of canine astroviruses. Journal of General Virology, 92(8), 1880-1887. https://doi.org/10.1099/vir.0.029025-0

Detection and characterization of canine astroviruses. / Martella, Vito; Moschidou, Paschalina; Lorusso, Eleonora; Mari, Viviana; Camero, Michele; Bellacicco, Annalucia; Losurdo, Michele; Pinto, Pierfrancesco; Desario, Costantina; Bányai, K.; Elia, Gabriella; Decaro, Nicola; Buonavoglia, Canio.

In: Journal of General Virology, Vol. 92, No. 8, 08.2011, p. 1880-1887.

Research output: Contribution to journalArticle

Martella, V, Moschidou, P, Lorusso, E, Mari, V, Camero, M, Bellacicco, A, Losurdo, M, Pinto, P, Desario, C, Bányai, K, Elia, G, Decaro, N & Buonavoglia, C 2011, 'Detection and characterization of canine astroviruses', Journal of General Virology, vol. 92, no. 8, pp. 1880-1887. https://doi.org/10.1099/vir.0.029025-0
Martella V, Moschidou P, Lorusso E, Mari V, Camero M, Bellacicco A et al. Detection and characterization of canine astroviruses. Journal of General Virology. 2011 Aug;92(8):1880-1887. https://doi.org/10.1099/vir.0.029025-0
Martella, Vito ; Moschidou, Paschalina ; Lorusso, Eleonora ; Mari, Viviana ; Camero, Michele ; Bellacicco, Annalucia ; Losurdo, Michele ; Pinto, Pierfrancesco ; Desario, Costantina ; Bányai, K. ; Elia, Gabriella ; Decaro, Nicola ; Buonavoglia, Canio. / Detection and characterization of canine astroviruses. In: Journal of General Virology. 2011 ; Vol. 92, No. 8. pp. 1880-1887.
@article{abbaf762169d44a3a29bc0c765ff2007,
title = "Detection and characterization of canine astroviruses",
abstract = "Astroviruses (AstVs) have been identified only occasionally in dogs. A canine AstV, strain Bari/ 08/ITA, was detected from a pup with gastroenteric signs and the virus was isolated in cell culture and characterized molecularly. In the full-length capsid protein, the virus displayed genetic similarities (83.5{\%} aa identity) to another canine AstV strain, although a high rate of variation occurred in the hypervariable domain, which is related to AstV antigenic specificity. Specific antibodies were detected in the convalescent dog, indicating seroconversion, and in 59{\%} of a collection of dog serum samples. Using primers specific for canine AstV, designed to detect a conserved region of ORF1b, canine AstVs were detected in 24.5{\%} of young pups with gastroenteritis, either alone or in mixed infections with other canine pathogens. In contrast, AstVs were detected in only 9.3{\%} of asymptomatic pups. These findings indicate that canine AstVs are common in dogs and may suggest a possible role as canine enteric pathogens.",
author = "Vito Martella and Paschalina Moschidou and Eleonora Lorusso and Viviana Mari and Michele Camero and Annalucia Bellacicco and Michele Losurdo and Pierfrancesco Pinto and Costantina Desario and K. B{\'a}nyai and Gabriella Elia and Nicola Decaro and Canio Buonavoglia",
year = "2011",
month = "8",
doi = "10.1099/vir.0.029025-0",
language = "English",
volume = "92",
pages = "1880--1887",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "8",

}

TY - JOUR

T1 - Detection and characterization of canine astroviruses

AU - Martella, Vito

AU - Moschidou, Paschalina

AU - Lorusso, Eleonora

AU - Mari, Viviana

AU - Camero, Michele

AU - Bellacicco, Annalucia

AU - Losurdo, Michele

AU - Pinto, Pierfrancesco

AU - Desario, Costantina

AU - Bányai, K.

AU - Elia, Gabriella

AU - Decaro, Nicola

AU - Buonavoglia, Canio

PY - 2011/8

Y1 - 2011/8

N2 - Astroviruses (AstVs) have been identified only occasionally in dogs. A canine AstV, strain Bari/ 08/ITA, was detected from a pup with gastroenteric signs and the virus was isolated in cell culture and characterized molecularly. In the full-length capsid protein, the virus displayed genetic similarities (83.5% aa identity) to another canine AstV strain, although a high rate of variation occurred in the hypervariable domain, which is related to AstV antigenic specificity. Specific antibodies were detected in the convalescent dog, indicating seroconversion, and in 59% of a collection of dog serum samples. Using primers specific for canine AstV, designed to detect a conserved region of ORF1b, canine AstVs were detected in 24.5% of young pups with gastroenteritis, either alone or in mixed infections with other canine pathogens. In contrast, AstVs were detected in only 9.3% of asymptomatic pups. These findings indicate that canine AstVs are common in dogs and may suggest a possible role as canine enteric pathogens.

AB - Astroviruses (AstVs) have been identified only occasionally in dogs. A canine AstV, strain Bari/ 08/ITA, was detected from a pup with gastroenteric signs and the virus was isolated in cell culture and characterized molecularly. In the full-length capsid protein, the virus displayed genetic similarities (83.5% aa identity) to another canine AstV strain, although a high rate of variation occurred in the hypervariable domain, which is related to AstV antigenic specificity. Specific antibodies were detected in the convalescent dog, indicating seroconversion, and in 59% of a collection of dog serum samples. Using primers specific for canine AstV, designed to detect a conserved region of ORF1b, canine AstVs were detected in 24.5% of young pups with gastroenteritis, either alone or in mixed infections with other canine pathogens. In contrast, AstVs were detected in only 9.3% of asymptomatic pups. These findings indicate that canine AstVs are common in dogs and may suggest a possible role as canine enteric pathogens.

UR - http://www.scopus.com/inward/record.url?scp=79960366804&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960366804&partnerID=8YFLogxK

U2 - 10.1099/vir.0.029025-0

DO - 10.1099/vir.0.029025-0

M3 - Article

C2 - 21471316

AN - SCOPUS:79960366804

VL - 92

SP - 1880

EP - 1887

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 8

ER -