Detection and characterization of canine astroviruses

Vito Martella, Paschalina Moschidou, Eleonora Lorusso, Viviana Mari, Michele Camero, Annalucia Bellacicco, Michele Losurdo, Pierfrancesco Pinto, Costantina Desario, Kristian Bányai, Gabriella Elia, Nicola Decaro, Canio Buonavoglia

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Astroviruses (AstVs) have been identified only occasionally in dogs. A canine AstV, strain Bari/ 08/ITA, was detected from a pup with gastroenteric signs and the virus was isolated in cell culture and characterized molecularly. In the full-length capsid protein, the virus displayed genetic similarities (83.5% aa identity) to another canine AstV strain, although a high rate of variation occurred in the hypervariable domain, which is related to AstV antigenic specificity. Specific antibodies were detected in the convalescent dog, indicating seroconversion, and in 59% of a collection of dog serum samples. Using primers specific for canine AstV, designed to detect a conserved region of ORF1b, canine AstVs were detected in 24.5% of young pups with gastroenteritis, either alone or in mixed infections with other canine pathogens. In contrast, AstVs were detected in only 9.3% of asymptomatic pups. These findings indicate that canine AstVs are common in dogs and may suggest a possible role as canine enteric pathogens.

Original languageEnglish
Pages (from-to)1880-1887
Number of pages8
JournalJournal of General Virology
Volume92
Issue number8
DOIs
Publication statusPublished - Aug 1 2011

ASJC Scopus subject areas

  • Virology

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    Martella, V., Moschidou, P., Lorusso, E., Mari, V., Camero, M., Bellacicco, A., Losurdo, M., Pinto, P., Desario, C., Bányai, K., Elia, G., Decaro, N., & Buonavoglia, C. (2011). Detection and characterization of canine astroviruses. Journal of General Virology, 92(8), 1880-1887. https://doi.org/10.1099/vir.0.029025-0