Detection and analysis of transpositionally active head-to-tail dimers in three additional Escherichia coli IS elements

Ildikó Szeverényi, Zita Nagy, Tibor Farkas, F. Olasz, János Kiss

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

This study demonstrates that Escherichia coli insertion elements IS3, IS150 and IS186 are able to form transpositionally active head-to-tail dimers which show similar structure and transpositional activity to the dimers of IS2, IS21 and IS30. These structures arise by joining of the left and right inverted repeats (IRs) of two elements. The resulting junction includes a spacer region (SR) of a few base pairs derived from the flanking sequence of one of the reacting IRs. Head-to-tail dimers of IS3, IS150 and IS186 are unstable due to their transpositional activity. They can be resolved in two ways that seem to form a general rule for those elements reported to form dimers. One way is a site-specific process (dimer dissolution) which is accompanied by the loss of one IS copy along with the SR. The other is 'classical' transposition where the joined ends integrate into the target DNA. In intramolecular transposition this often gives rise to deletion formation, whereas in intermolecular transposition it gives rise to replicon fusion. The results presented for IS3, IS150 and IS186 are in accordance with the IS dimer model, which is in turn consistent with models based on covalently closed minicircles.

Original languageEnglish
Pages (from-to)1297-1310
Number of pages14
JournalMicrobiology
Volume149
Issue number5
DOIs
Publication statusPublished - May 1 2003

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Replicon
DNA Transposable Elements
Base Pairing
Head
Escherichia coli
DNA

ASJC Scopus subject areas

  • Microbiology

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Detection and analysis of transpositionally active head-to-tail dimers in three additional Escherichia coli IS elements. / Szeverényi, Ildikó; Nagy, Zita; Farkas, Tibor; Olasz, F.; Kiss, János.

In: Microbiology, Vol. 149, No. 5, 01.05.2003, p. 1297-1310.

Research output: Contribution to journalArticle

Szeverényi, Ildikó ; Nagy, Zita ; Farkas, Tibor ; Olasz, F. ; Kiss, János. / Detection and analysis of transpositionally active head-to-tail dimers in three additional Escherichia coli IS elements. In: Microbiology. 2003 ; Vol. 149, No. 5. pp. 1297-1310.
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