Detection and analysis of six lizard adenoviruses by consensus primer PCR provides further evidence of a reptilian origin for the atadenoviruses

James F.X. Wellehan, April J. Johnson, Balázs Harrach, Mária Benkö, Allan P. Pessier, Calvin M. Johnson, Michael M. Garner, April Childress, Elliott R. Jacobson

Research output: Contribution to journalArticle

196 Citations (Scopus)

Abstract

A consensus nested-PCR method was designed for investigation of the DNA polymerase gene of adenoviruses. Gene fragments were amplified and sequenced from six novel adenoviruses from seven lizard species, including four species from which adenoviruses had not previously been reported. Host species included Gila monster, leopard gecko, fat-tail gecko, blue-tongued skink, Tokay gecko, bearded dragon, and mountain chameleon. This is the first sequence information from lizard adenoviruses. Phylogenetic analysis indicated that these viruses belong to the genus Atadenovirus, supporting the reptilian origin of atadenoviruses. This PCR method may be useful for obtaining templates for initial sequencing of novel adenoviruses.

Original languageEnglish
Pages (from-to)13366-13369
Number of pages4
JournalJournal of Virology
Volume78
Issue number23
DOIs
Publication statusPublished - Dec 2004

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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