Detecting native folds in mixtures of proteins that contain disulfide bonds

Mahesh Narayan, E. Welker, Huili Zhai, Xuemei Han, Guoqiang Xu, Fred W. McLafferty, Harold A. Scheraga

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

High-throughput in vitro refolding of proteins that contain disulfide bonds, for which soluble expression is particularly difficult, is severely impeded by the absence of effective methods for detecting their native forms. We demonstrate such a method, which combines mass spectrometry with mild reductions, requires no prior experimentation or knowledge of proteins' physicochemical characteristics, function or activity, and is amenable to automation. These are necessary criteria for structural genomics and proteomics applications.

Original languageEnglish
Pages (from-to)427-429
Number of pages3
JournalNature Biotechnology
Volume26
Issue number4
DOIs
Publication statusPublished - Apr 2008

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Disulfides
Protein Refolding
Proteins
Automation
Genomics
Proteomics
Mass spectrometry
Mass Spectrometry
Throughput
In Vitro Techniques

ASJC Scopus subject areas

  • Microbiology

Cite this

Narayan, M., Welker, E., Zhai, H., Han, X., Xu, G., McLafferty, F. W., & Scheraga, H. A. (2008). Detecting native folds in mixtures of proteins that contain disulfide bonds. Nature Biotechnology, 26(4), 427-429. https://doi.org/10.1038/nbt1380

Detecting native folds in mixtures of proteins that contain disulfide bonds. / Narayan, Mahesh; Welker, E.; Zhai, Huili; Han, Xuemei; Xu, Guoqiang; McLafferty, Fred W.; Scheraga, Harold A.

In: Nature Biotechnology, Vol. 26, No. 4, 04.2008, p. 427-429.

Research output: Contribution to journalArticle

Narayan, M, Welker, E, Zhai, H, Han, X, Xu, G, McLafferty, FW & Scheraga, HA 2008, 'Detecting native folds in mixtures of proteins that contain disulfide bonds', Nature Biotechnology, vol. 26, no. 4, pp. 427-429. https://doi.org/10.1038/nbt1380
Narayan, Mahesh ; Welker, E. ; Zhai, Huili ; Han, Xuemei ; Xu, Guoqiang ; McLafferty, Fred W. ; Scheraga, Harold A. / Detecting native folds in mixtures of proteins that contain disulfide bonds. In: Nature Biotechnology. 2008 ; Vol. 26, No. 4. pp. 427-429.
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