Detailed analyses of antibodies recognizing mitochondrial antigens suggest similar or identical mechanism for production of natural antibodies and natural autoantibodies

T. Czömpöly, Katalin Olasz, Zoltán Nyárády, Diána Simon, Judit Bovári, P. Németh

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Because of their endosymbiotic evolutionary origin, proteins compartmentalized into mitochondria represent an interesting transition from prokaryotic foreign to essential self molecules. We investigated the presence of naturally occurring antibodies (nAbs) recognizing mitochondrial inner membrane enzymes. Epitope mapping analysis of a mitochondrial inner membrane enzyme, citrate synthase (CS) by synthetic overlapping peptides and phage display libraries using sera from healthy individuals and from patients having systemic autoimmune disease revealed CS recognizing nAbs with IgM isotype. We analyzed cross-reactive epitopes on human CS, bacterial CS, and various standard autoantigens. We have found that the fine epitope pattern on CS is different under physiological and pathological conditions. Moreover sera affinity purified on CS cross reacts with nucleosome antigen, which cross-reactivity could be mapped to a short epitope on human CS. These data indicate that in theory, nAbs "specific" for a given self antigen could fulfill the function of participating in innate defense mechanisms and at the same time recognize a target antigen in a systemic autoimmune disease. Thus, at the level of recognized epitopes there is a possible link between the innate like part and the adaptive-autoimmune arm of the humoral immune system.

Original languageEnglish
Pages (from-to)463-467
Number of pages5
JournalAutoimmunity Reviews
Volume7
Issue number6
DOIs
Publication statusPublished - Jun 2008

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Citrate (si)-Synthase
Autoantibodies
Antibody Formation
Antigens
Antibodies
Epitopes
Autoantigens
Mitochondrial Membranes
Autoimmune Diseases
Epitope Mapping
Peptide Library
Nucleosomes
Enzymes
Serum
Immunoglobulin M
Immune System
Mitochondria

Keywords

  • Epitope mapping
  • Mitochondrial inner membrane enzyme
  • Naturally occurring antibodies

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Detailed analyses of antibodies recognizing mitochondrial antigens suggest similar or identical mechanism for production of natural antibodies and natural autoantibodies. / Czömpöly, T.; Olasz, Katalin; Nyárády, Zoltán; Simon, Diána; Bovári, Judit; Németh, P.

In: Autoimmunity Reviews, Vol. 7, No. 6, 06.2008, p. 463-467.

Research output: Contribution to journalArticle

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