Design and operation of a bench-scale process development unit for the production of ethanol from lignocellulosics

Eva Palmqvist, Bärbel Hahn-Hägerdal, Mats Galbe, Mats Larsson, Kerstin Stenberg, Zs. Szengyel, Charlotte Tengborg, Guido Zacchi

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

A bench-scale unit for the development of an enzymatic process for the bioconversion of lignocellulosics to ethanol has been used to study the recycling of waste-water streams to minimize fresh-water requirements and reduce effluent streams. Willow, after impregnation with sulphur dioxide, was sream-pretreated, enzymatically hydrolysed, and the sugars produced were fermented using S. cerevisiae. The fermentation broth was distilled and the stillage was fractionated by evaporation into six separate condensate fractions and a residue. The overall yield of ethanol from willow was 65% of the theoretical yield based on total fermentable sugars. The inhibitory effect of the evaporation condensates was assessed by fermentation using S. cerevisiae. The non-volatile residue of the stillage was found to be inhibitory to fermentation. The ethanol yield decreased from 0.37 g/g in a pure sugar reference to 0.31 g/g in the residue and the average ethanol fermentation rate decreased from 6.3 g/(lh) to 2.7 g/(l h), respectively. The evaporation condensates, containing the volatile components, showed no negative effects on fermentation. The intermediate evaporation condensate fractions, fractions 4 and 5, had the lowest chemical oxygen demand (COD), 1560 and 1120 mg/l, compared with 33300 mg/l for the stillage. Therefore, these fractions can be released directly into the effluent without further treatment.

Original languageEnglish
Pages (from-to)171-179
Number of pages9
JournalBioresource Technology
Volume58
Issue number2
DOIs
Publication statusPublished - Nov 1996

Fingerprint

ethanol production
Fermentation
fermentation
evaporation
ethanol
condensate
Ethanol
Evaporation
Sugars
Salix
sugars
sugar
effluents
Saccharomyces cerevisiae
Effluents
ethanol fermentation
chemical oxygen demand
effluent
water requirement
Biological Oxygen Demand Analysis

Keywords

  • Enzymatic process
  • Ethanol production
  • Inhibitors
  • Lignocellulosics
  • Steam treatment
  • Waste-water recycling
  • Willow

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Food Science
  • Process Chemistry and Technology
  • Applied Microbiology and Biotechnology

Cite this

Design and operation of a bench-scale process development unit for the production of ethanol from lignocellulosics. / Palmqvist, Eva; Hahn-Hägerdal, Bärbel; Galbe, Mats; Larsson, Mats; Stenberg, Kerstin; Szengyel, Zs.; Tengborg, Charlotte; Zacchi, Guido.

In: Bioresource Technology, Vol. 58, No. 2, 11.1996, p. 171-179.

Research output: Contribution to journalArticle

Palmqvist, E, Hahn-Hägerdal, B, Galbe, M, Larsson, M, Stenberg, K, Szengyel, Z, Tengborg, C & Zacchi, G 1996, 'Design and operation of a bench-scale process development unit for the production of ethanol from lignocellulosics', Bioresource Technology, vol. 58, no. 2, pp. 171-179. https://doi.org/10.1016/S0960-8524(96)00096-X
Palmqvist, Eva ; Hahn-Hägerdal, Bärbel ; Galbe, Mats ; Larsson, Mats ; Stenberg, Kerstin ; Szengyel, Zs. ; Tengborg, Charlotte ; Zacchi, Guido. / Design and operation of a bench-scale process development unit for the production of ethanol from lignocellulosics. In: Bioresource Technology. 1996 ; Vol. 58, No. 2. pp. 171-179.
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