Desiccoplast-etioplast-chloroplast transformation under rehydration of desiccated poikilochlorophyllous Xerophyta humilis leaves in the dark and upon subsequent illumination

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8 Citations (Scopus)

Abstract

The transformation of desiccoplasts into etioplasts and the parallel appearance of protochlorophyllide (Pchlide) forms were observed with transmission electron microscopy and 77. K fluorescence spectroscopy, when air-dried detached leaves of the poikilochlorophyllous desiccation tolerant plant Xerophyta humilis were floated in water in the dark. After 1 week of rehydration, pregranal plastids with newly synthesized prothylakoid (PT) lamellae and mainly non-photoactive Pchlide forms developed, while etioplasts with prolamellar bodies (PLBs) and photoactive, 655. nm emitting Pchlide form accumulated primarily in the basal leaf regions after 2 weeks of regeneration. When these latter leaves were illuminated with continuous light for 3 days, the etioplasts transformed into regular chloroplasts and the fluorescence emission bands characteristic of green leaves appeared. These results show that, upon rehydration, the dehydrated chlorenchyma cells are able to regenerate pregranal plastids and etioplasts from desiccoplasts in the dark, which can transform into regular chloroplasts when they are illuminated. This means that the differentiation of pregranal plastids and etioplasts and their greening process is a basic property of fully differentiated cells of X. humilis. Consequently, these processes are not merely characteristic for seedlings with meristematic and differentiating young tissues.

Original languageEnglish
Pages (from-to)583-590
Number of pages8
JournalJournal of Plant Physiology
Volume170
Issue number6
DOIs
Publication statusPublished - Apr 15 2013

Fingerprint

etioplasts
Fluid Therapy
rehydration
Chloroplasts
Lighting
lighting
protochlorophyllides
chloroplasts
Protochlorophyllide
plastids
Plastids
leaves
fluorescence emission spectroscopy
chlorenchyma
air drying
Desiccation
transmission electron microscopy
Fluorescence Spectrometry
Transmission Electron Microscopy
fluorescence

Keywords

  • Chlorophyll biosynthesis
  • Etioplast
  • Poikilochlorophyllous desiccation-tolerant plant
  • Protochlorophyllide
  • Rehydration

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Agronomy and Crop Science

Cite this

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title = "Desiccoplast-etioplast-chloroplast transformation under rehydration of desiccated poikilochlorophyllous Xerophyta humilis leaves in the dark and upon subsequent illumination",
abstract = "The transformation of desiccoplasts into etioplasts and the parallel appearance of protochlorophyllide (Pchlide) forms were observed with transmission electron microscopy and 77. K fluorescence spectroscopy, when air-dried detached leaves of the poikilochlorophyllous desiccation tolerant plant Xerophyta humilis were floated in water in the dark. After 1 week of rehydration, pregranal plastids with newly synthesized prothylakoid (PT) lamellae and mainly non-photoactive Pchlide forms developed, while etioplasts with prolamellar bodies (PLBs) and photoactive, 655. nm emitting Pchlide form accumulated primarily in the basal leaf regions after 2 weeks of regeneration. When these latter leaves were illuminated with continuous light for 3 days, the etioplasts transformed into regular chloroplasts and the fluorescence emission bands characteristic of green leaves appeared. These results show that, upon rehydration, the dehydrated chlorenchyma cells are able to regenerate pregranal plastids and etioplasts from desiccoplasts in the dark, which can transform into regular chloroplasts when they are illuminated. This means that the differentiation of pregranal plastids and etioplasts and their greening process is a basic property of fully differentiated cells of X. humilis. Consequently, these processes are not merely characteristic for seedlings with meristematic and differentiating young tissues.",
keywords = "Chlorophyll biosynthesis, Etioplast, Poikilochlorophyllous desiccation-tolerant plant, Protochlorophyllide, Rehydration",
author = "K. Solymosi and Z. Tuba and B. B{\"o}ddi",
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T1 - Desiccoplast-etioplast-chloroplast transformation under rehydration of desiccated poikilochlorophyllous Xerophyta humilis leaves in the dark and upon subsequent illumination

AU - Solymosi, K.

AU - Tuba, Z.

AU - Böddi, B.

PY - 2013/4/15

Y1 - 2013/4/15

N2 - The transformation of desiccoplasts into etioplasts and the parallel appearance of protochlorophyllide (Pchlide) forms were observed with transmission electron microscopy and 77. K fluorescence spectroscopy, when air-dried detached leaves of the poikilochlorophyllous desiccation tolerant plant Xerophyta humilis were floated in water in the dark. After 1 week of rehydration, pregranal plastids with newly synthesized prothylakoid (PT) lamellae and mainly non-photoactive Pchlide forms developed, while etioplasts with prolamellar bodies (PLBs) and photoactive, 655. nm emitting Pchlide form accumulated primarily in the basal leaf regions after 2 weeks of regeneration. When these latter leaves were illuminated with continuous light for 3 days, the etioplasts transformed into regular chloroplasts and the fluorescence emission bands characteristic of green leaves appeared. These results show that, upon rehydration, the dehydrated chlorenchyma cells are able to regenerate pregranal plastids and etioplasts from desiccoplasts in the dark, which can transform into regular chloroplasts when they are illuminated. This means that the differentiation of pregranal plastids and etioplasts and their greening process is a basic property of fully differentiated cells of X. humilis. Consequently, these processes are not merely characteristic for seedlings with meristematic and differentiating young tissues.

AB - The transformation of desiccoplasts into etioplasts and the parallel appearance of protochlorophyllide (Pchlide) forms were observed with transmission electron microscopy and 77. K fluorescence spectroscopy, when air-dried detached leaves of the poikilochlorophyllous desiccation tolerant plant Xerophyta humilis were floated in water in the dark. After 1 week of rehydration, pregranal plastids with newly synthesized prothylakoid (PT) lamellae and mainly non-photoactive Pchlide forms developed, while etioplasts with prolamellar bodies (PLBs) and photoactive, 655. nm emitting Pchlide form accumulated primarily in the basal leaf regions after 2 weeks of regeneration. When these latter leaves were illuminated with continuous light for 3 days, the etioplasts transformed into regular chloroplasts and the fluorescence emission bands characteristic of green leaves appeared. These results show that, upon rehydration, the dehydrated chlorenchyma cells are able to regenerate pregranal plastids and etioplasts from desiccoplasts in the dark, which can transform into regular chloroplasts when they are illuminated. This means that the differentiation of pregranal plastids and etioplasts and their greening process is a basic property of fully differentiated cells of X. humilis. Consequently, these processes are not merely characteristic for seedlings with meristematic and differentiating young tissues.

KW - Chlorophyll biosynthesis

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