Desensitization of luteinizing hormone release in cultured pituitary cells by gonadotropin-releasing hormone

G. Kéri, Károly Nikolics, István Teplán, Judit Molnár

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Preincubation of cultured pituitary cells with GnRH caused a marked decrease in subsequent LH release. The rate of desensitization increased when the preincubating concentration of GnRH and the preincubation time were increased. Pituitary cells obtained from male rats were not as sensitive to GnRH as cells obtained from female rats and the extent of desensitization was also smaller in cells from male rats. Desensitization was found to be a long-lasting effect, without any change in the viability of the cells. A superactive analogue of GnRH (D-Phe6-GnRH) caused almost complete desensitization of LH secretion, while a competitive inhibitory analogue of GnRH caused a much smaller decrease in LH response which could be overcome by increasing the concentration of GnRH used for reincubation. These data suggest that the desensitization is closely related to the biological activity of GnRH and does not correlate with receptor binding. High concentrations of potassium also induced desensitization, although to a lower extent than GnRH. Since K+ induces LH release by a different mechanism than GnRH, our data suggest that the desensitization phenomenon cannot be explained only at the receptor level. The time curve of desensitization supports the idea that GnRH action has two phases: an acute effect which cannot be desensitized, and a secondary phase which can be desensitized.

Original languageEnglish
Pages (from-to)109-120
Number of pages12
JournalMolecular and Cellular Endocrinology
Volume30
Issue number1
DOIs
Publication statusPublished - 1983

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Pituitary Gonadotropins
Luteinizing Hormone
Gonadotropin-Releasing Hormone
Cultured Cells
Rats
Bioactivity
Cell Survival
Potassium
Cells

Keywords

  • desensitization
  • GnRH
  • LH release
  • pituitary cell culture

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Desensitization of luteinizing hormone release in cultured pituitary cells by gonadotropin-releasing hormone. / Kéri, G.; Nikolics, Károly; Teplán, István; Molnár, Judit.

In: Molecular and Cellular Endocrinology, Vol. 30, No. 1, 1983, p. 109-120.

Research output: Contribution to journalArticle

Kéri, G. ; Nikolics, Károly ; Teplán, István ; Molnár, Judit. / Desensitization of luteinizing hormone release in cultured pituitary cells by gonadotropin-releasing hormone. In: Molecular and Cellular Endocrinology. 1983 ; Vol. 30, No. 1. pp. 109-120.
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