Description of the nicotiana benthamiana-cercospora nicotianae pathosystem

J. Fodor, Evelin Kámán-Tóth, Tamás Dankó, Ildikó Schwarczinger, Zoltan Bozsó, Miklós Pogány

Research output: Contribution to journalArticle

Abstract

Nicotiana benthamiana is a valuable model organism in plant biology research. This report describes its extended applicability in the field of molecular plant pathology by introducing a nonbiotrophic fungal pathogen Cercospora nicotianae that can be conveniently used under laboratory conditions, consistently induces a necrotic leaf spot disease on Nicotiana benthamiana, and is specialized on solanaceous plants. Our inoculation studies showed that C. nicotianae more effectively colonizes N. benthamiana than its conventional host, N. tabacum. The functions of two critical regulators of host immunity, coronatine-insensitive 1 (COI1) and ethylene-insensitive 2 (EIN2), were studied in N. benthamiana using Tobacco rattle virus-based virus-induced gene silencing (VIGS). Perturbation of jasmonic acid or ethylene signaling by VIGS of either COI1 or EIN2, respectively, resulted in markedly increased Cercospora leaf spot symptoms on N. benthamiana plants. These results suggest that the N. benthamiana- C. nicotianae host-pathogen interaction is a prospective but hitherto unutilized pathosystem for studying gene functions in diseased plants.

Original languageEnglish
Pages (from-to)149-155
Number of pages7
JournalPhytopathology
Volume108
Issue number1
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Cercospora nicotianae
Nicotiana benthamiana
leaf spot
ethylene
gene silencing
Tobacco rattle virus
Cercospora
viruses
host-pathogen relationships
plant pathology
plant biology
jasmonic acid
signs and symptoms (plants)
immunity
pathogens
organisms

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Fodor, J., Kámán-Tóth, E., Dankó, T., Schwarczinger, I., Bozsó, Z., & Pogány, M. (2018). Description of the nicotiana benthamiana-cercospora nicotianae pathosystem. Phytopathology, 108(1), 149-155. https://doi.org/10.1094/PHYTO-12-16-0448-R

Description of the nicotiana benthamiana-cercospora nicotianae pathosystem. / Fodor, J.; Kámán-Tóth, Evelin; Dankó, Tamás; Schwarczinger, Ildikó; Bozsó, Zoltan; Pogány, Miklós.

In: Phytopathology, Vol. 108, No. 1, 01.01.2018, p. 149-155.

Research output: Contribution to journalArticle

Fodor, J, Kámán-Tóth, E, Dankó, T, Schwarczinger, I, Bozsó, Z & Pogány, M 2018, 'Description of the nicotiana benthamiana-cercospora nicotianae pathosystem', Phytopathology, vol. 108, no. 1, pp. 149-155. https://doi.org/10.1094/PHYTO-12-16-0448-R
Fodor J, Kámán-Tóth E, Dankó T, Schwarczinger I, Bozsó Z, Pogány M. Description of the nicotiana benthamiana-cercospora nicotianae pathosystem. Phytopathology. 2018 Jan 1;108(1):149-155. https://doi.org/10.1094/PHYTO-12-16-0448-R
Fodor, J. ; Kámán-Tóth, Evelin ; Dankó, Tamás ; Schwarczinger, Ildikó ; Bozsó, Zoltan ; Pogány, Miklós. / Description of the nicotiana benthamiana-cercospora nicotianae pathosystem. In: Phytopathology. 2018 ; Vol. 108, No. 1. pp. 149-155.
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