Derivatization and chromatographic behavior of the o-phthaldialdehyde amino acid derivatives obtained with various SH-group-containing additives

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Abstract

An overview is presented of HPLC methods currently in use to determine amino acids as their o-phthaldialdyde derivatives in the presence of various SH-group-containing additives. Crucial points that proved to influence the stability of the amino acid OPA derivatives have been discussed in detail: (i) the mol ratios of the OPA-SH-group-containing additive amino acid; (ii) the preparation and storage conditions of the OPA reagents; (iii) the optimum pH conditions for the interactions and elutions; (iv) the behavior of the, believed to be, less stable amino acids, such as glycine, β-alanine, γ-aminobutyric acid, histidine, ornithine and lysine.

Original languageEnglish
Pages (from-to)283-302
Number of pages20
JournalJournal of Chromatography A
Volume913
Issue number1-2
DOIs
Publication statusPublished - Apr 13 2001

Keywords

  • Amino acids
  • Amino acids, OPA derivatives
  • Derivatization, LC
  • Reviews

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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